摘要： 使用聚丙烯酰胺凝胶垂直平板电泳法，研究了不同浓度（10^-4~10^-8mol/L）的Cd²⁺，Ni²⁺和Hg²⁺对大麦根尖细胞过氧化物酶同工酶的影响。结果表明，10^-4mol/LCd²⁺和Hg²⁺处理24h均比对照增加1条酶带（Rf=0.36），但前者缺失了1条Rf值为0.80的酶带8。处理48h后，10^-6mol/L的Hg2 + 处理组又增加1条Rf值为0.36的新酶带。其余浓度的Cd²⁺，Hg²⁺和3种浓度的Ni²⁺均可使同工酶强弱发生改变。Cd²⁺和Hg²⁺对过氧化酶的影响明显大于Ni²⁺。3种重金属对大麦过氧化物酶同工酶的影响与浓度和处理时间有关。

关键词: 镉, 镍, 汞, 过氧化物酶同工酶, 大麦

Abstract: By using vertical polyacrylamide gel electrophoresis, the effects of three heavy metals at different concentrations on the peroxidase isozymes of barley root cells were studied. The results indicated that after 24 h treatment with 10^-4mol/LCd²⁺ and Hg²⁺, one additional band (Rf=0.36) increased for each treatment, but a band (Rf=0.80) was missing in the former comparison with the control. After the treatment with 10^-6mol/L Hg²⁺ for 48h, the treatment group added another band (Rf=0.36). The activites of peroxidase isozymes were changed greatly at rest concentrations ofCd²⁺、Hg²⁺ and 10^-4~10^-8mol/L Ni²⁺ treatment groups. It suggested that Hg²⁺ andCd²⁺ have stronger effects on the peroxidase isozymes than Ni²⁺ and the degree of effects of heavy metals on the peroxidase isozymes is dependent on their concentrations and treatment durations.

Key words: Cadmium, Nickel, Mercury, Peroxidase isozymes, Hordeum vulgare

中图分类号: 

• S512.301