摘要： 以陆地棉品种“珂字201”为材料，比较了IAA+KT和2，4-D+KT在愈伤诱导和悬浮培养中的效应，结果表明，愈伤组织诱导中，IAA和2，4-D表现为正效应，且2，4-D的效应强于IAA；KT表现为负效应；胚性愈伤悬浮培养中，3种激素都表现出负效应。以胚性细胞悬浮系为材料进行了原生质体的分离和培养试验，分离原生质体的最佳酶组合为纤维素酶（Onozuka R10）3%+果胶酶（Pectinase）1.5%，原生质体培养的最佳激素组合为IAA 0.5 mg/L+KT 0·1 mg/L，适当提高原生质体密度有利于原生质体培养。光照，温度等物理因素对原生质体培养也有明显影响。原生质体再生的愈伤组织直接在无激素的悬浮培养基中增殖，很快获得了大量胚性愈伤组织，经分化培养再生出植株。

关键词: 陆地棉, 原生质体培养, 植株再生

Abstract: In callus induction of Gossypium hirsutum L.cv.coker 201, IAA and 2, 4-D showed positive effect and KT showed negative effect, while negative effect was shown with the three hormones in suspensions.The best enzymatic combination in protoplast isolation from embryogenetic suspensions was 3% cellulase(Onozuka R10)+1.5%pectinase, and the best hormone combination in protoplast culture was IAA 0.5mg/L+KT 0.1mg/L. Appropriately increasing protoplast density benefited protoplast culture. Light and temprature affected the protoplast culture.Protoplast-derived calli growed rapidly in suspension culture medium without any hormone and regenerated plants through differentiation culture.

Key words: Gossypium hirsutum L, Protoplast culture, Plant regeneration