陆地棉<em>GhZIP</em>基因家族全基因组分析

doi:10.7668/hbnxb.2015.06.002

摘要/Abstract

摘要： ZIP 基因家族是生物体内非常重要的一类金属阳离子转运蛋白基因家族,通过对陆地棉基因组的全面分析和鉴定,获得GhZIP 基因家族系统性的生物学信息.运用比较基因组学的方法,从陆地棉异源四倍体标准系TM-1基因组数据库中鉴定出45个GhZIP 基因,这些基因分布在除了A04、A09、D04和D09号亚基因组以外的22个亚基因组,D亚组比A亚组多5个GhZIP 基因,有16对基因在A亚组和相对的D亚组上存在一一对应关系.有44个GhZIP 基因具有跨膜结构,其中有36个GhZIP 基因的跨膜结构域是6~9个,具有His富集区序列并位于膜内侧可变区的有20个GhZIP 基因,只有1个GhZIP 基因亚细胞定位不在细胞质膜.对NCBI 中陆地棉的EST数据库做比对统计,有21个GhZIP 基因没有可匹配的EST序列,其他24个GhZIP 基因在棉花的根、茎、叶、花、胚珠、纤维等组织中广泛表达,其中有18个基因在根和/或茎中表达.研究结果为GhZIP 基因的克隆和功能研究提供了理论基础.

关键词: 棉花, 陆地棉基因组, GhZIP 基因

Abstract: ZIP gene family is one of the most important metal cation transporter gene family within the organism.This work is to obtain the systematic biological information of GhZIP gene family through comprehensive analysis and identification of the Gossypium hirsutum genome sequence.Comparative genomics approach was used throughout this research,45 GhZIP genes were identified from the genome sequence database of allotetraploid cotton ( Gossypium hirsutum L.acc.TM-1).This GhZIP genes were found to be distributed on 22 other subgenomes except for A04,A09,D04 and D09.D subgenomes was 5 more GhZIP genes than A subgenomes.There were 16 pairs of GhZIP genes in the A and D subgenomes with one-to-one correspondence relationship.Transmembrane domain was detected in 44 GhZIP genes,among them 36 GhZIP genes had 6-9 transmembrane domains.20 GhZIP genes had histidine-rich domain which localized at variable region of membrane inner.The result of subcellular localization indicated that only one GhZIP gene was not locate in plasmembrane.A Blast search on the NCBI EST database of Gossypium hirsutum showed that 21 GhZIP genes with no matching EST sequence,and the other 24 GhZIP genes were expressed in various tissues of root,stem,leaf,flower,ovules,fiber,et al.18 members in this 24 GhZIP genes were expressed in root and/or stem.The study results of this paper would provide an theoretical basis on cloning and functional research of GhZIP genes.

Key words: Cotton, Genome sequence of upland cotton, GhZIP gene

中图分类号:

S562.03
Q78

倪万潮, 巩元勇, 徐珍珍, 郭书巧, 束红梅, 蒋璐. 陆地棉GhZIP基因家族全基因组分析[J]. 华北农学报, 2015, 30(6): 8-16. doi: 10.7668/hbnxb.2015.06.002.

NI Wan-chao, GONG Yuan-yong, XU Zhen-zhen, GUO Shu-qiao, SHU Hong-mei, JIANG Lu. Genome-wide Analysis of the GhZIP Gene Family in Upland Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(6): 8-16. doi: 10.7668/hbnxb.2015.06.002.

http://www.hbnxb.net/CN/Y2015/V30/I6/8

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Genome-wide Analysis of the GhZIP Gene Family in Upland Cotton

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