陆地棉<em>EPSPS</em>基因全基因组分析

doi:10.7668/hbnxb.2016.01.003

华北农学报 ›› 2016, Vol. 31 ›› Issue (1): 15-21. doi: 10.7668/hbnxb.2016.01.003

所属专题： 棉花；生物技术；

陆地棉EPSPS基因全基因组分析

巩元勇, 徐珍珍, 郭书巧, 束红梅, 蒋璐, 倪万潮

江苏省农业科学院, 农业部长江下游棉花和油菜重点实验室, 江苏南京 210014

收稿日期:2015-11-09 出版日期:2016-02-28
通讯作者: 倪万潮(1962-),男,江苏盱眙人,研究员,硕士,主要从事生物技术研究。
作者简介:巩元勇(1982-),男,山东惠民人,副研究员,博士,主要从事植物基因分子生物学研究。
基金资助:
国家自然科学基金项目(31301682);江苏省农业科技自主创新基金项目(CX(14)5009);国家转基因重大专项(2013ZX08005)

Genome-wide Analysis of the EPSPS Genes in Upland Cotton

GONG Yuanyong, XU Zhenzhen, GUO Shuqiao, SHU Hongmei, JIANG Lu, NI Wanchao

Jiangsu Academy of Agricultural Sciences, Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Nanjing 210014, China

Received:2015-11-09 Published:2016-02-28

摘要/Abstract

摘要： 当前在NCBI中提交的来源于陆地棉的EPSPS基因有2个,随着陆地棉基因组测序结果的公布,为在陆地棉基因组中全面鉴定EPSPS基因的存在提供了便利条件。从陆地棉异源四倍体标准系TM-1基因组数据库中共搜寻获得4个EPSPS同源基因,这4个基因分别分布在A07、A12、D07和D12亚基因组。从这4个基因的核苷酸序列、氨基酸序列和基因结构的比对,构建进化树的系统发育分析以及基因的转录情况研究来看,定位在A07和D07亚基因组的2个基因属于共线性高度同源基因,定位在A12和D12亚基因组的2个基因也是相同的情况。序列比对结果表明,已经在NCBI中提交的2个EPSPS基因分别是定位在A12和D12亚基因组上的基因,研究结果为定位在A07和D07亚基因组上EPSPS基因的克隆和功能研究提供了基础理论依据。

关键词: 陆地棉, 陆地棉基因组, EPSPS基因

Abstract: Currently,two EPSPS genes from Gossypium hirsutum have been submitted to NCBI.It is more convinent to identify EPSPS genes in genome level of upland cotton comprehensively following the publication of Gossypium hirsutum genomic sequence.Four EPSPS homologous genes were identified from the genome sequence database of allotetraploid cotton (Gossypium hirsutum L.acc.TM-1),and they were found to be distributed on subgenomes of A07,A12,D07 and D12.Under the comperation of nucleotide sequences,amino acid sequences and gene structures,phylogenetic analysis of constructing phylogenetic tree and the study of transcription situation of these four genes,it was more clearly that the two genes located on A07 and D07 subgenomes belong to highly homologous co-linear gene,and the two genes located on A12 and D12 subgenomes as well.Sequence alignment results showed that the two EPSPS genes submitted to NCBI were the two genes located on A12 and D12 subgenomes respectively.The study results of this paper would provide an theoretical basis on cloning and functional research of EPSPS genes which located on A07 and D07 subgenomes.

Key words: Gossypium hirsutum, Genome sequence of upland cotton, EPSPS gene

中图分类号:

S562.01
Q78

巩元勇, 徐珍珍, 郭书巧, 束红梅, 蒋璐, 倪万潮. 陆地棉EPSPS基因全基因组分析[J]. 华北农学报, 2016, 31(1): 15-21. doi: 10.7668/hbnxb.2016.01.003.

GONG Yuanyong, XU Zhenzhen, GUO Shuqiao, SHU Hongmei, JIANG Lu, NI Wanchao. Genome-wide Analysis of the EPSPS Genes in Upland Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 15-21. doi: 10.7668/hbnxb.2016.01.003.

http://www.hbnxb.net/CN/Y2016/V31/I1/15

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