摘要： 利用花粉管通道以液滴法和喷雾法进行了谷子DNA导入玉米自交系齐302和478的试验研究。结果表明，两种方法在D₁均产生变异，导入组合变异率18.05%。在齐302变异中有一株雌穗顶端生长单枝雄穗且具分蘖、多穗特性的罕见变异。其它变异类型包括：生长势、株型、抗倒性、抗叶片衰老及分蘖和多穗变异。478只出现一个多穗变异，其长势旺盛，变异性状能够遗传至D₂，并有新性状分离，如穗行数、粒型、千粒重等变异。试验表明，外源DNA导入技术是创造新的玉米种质材料的有效方法。

关键词: 外源DNA导人, 玉米, 花粉管, 变异

Abstract: DNA from millet was.introduced into maize selfline and 478 by both droppingthe exogenous DNA on the excision of silks after selfpollination and spraying the exogenous DNA onto silks after selfpollination.In D,generation,the both methods caused variation.Therate of variation among the introduced combinations was 18.08%.There appeared a single planthving a tassel with single branch on the top of female ear with the characters of tiller and muitiale ears which never was found before.The other variation expressions were growth vigour,plantsh ape.loding resistance,leaf aging resistance,tiller and multiple ears.In 302 the combinationvaried and the variation rate of Lodging resistance was 16.40.Among 478 varied plants of D,genrration there appeared a multiple ear variation with strong growth vigour.All the variedrha.racters could be passed down to generation in which happened segregation of characters ineluding kernel rows per ear,1000kernel weight and kernel type.The experiment has suggestedthat the exogenous introduction techniqe is a good way of creating new materials for maizebreeding.

Key words: Exogenous DNA introduction, Maize, Pollen tube, Variation

中图分类号: 

• S336