华北农学报 ›› 1994, Vol. 9 ›› Issue (2): 59-63. doi: 10.3321/j.issn:1000-7091.1994.02.012

所属专题: 甜椒辣椒

• 论文 • 上一篇    下一篇

辣椒子叶离体培养再生植株

周钟信1, 张宗江1, 刘艳军1, 江倩云1, 米景九2   

  1. 1. 天津农学院园艺系生物工程研究室, 天津 300381;
    2. 北京农业大学生物学院, 100094
  • 收稿日期:1993-07-20 出版日期:1994-06-28

In vitro Plant Regeneration from Cotyledon Explants of Capsicum annuum

Zhou Zhongxin1, Zhang Zongjiang1, Liu Yianjun1, Jiang Qianyun1, Mi Jingjiu2   

  1. 1. Horticultural Department, Tianjin Agricultural College, Tianjin 300381;
    2. Biological College of Beijing Agricultural University, Beijing 100094
  • Received:1993-07-20 Published:1994-06-28

摘要: 随意收集到的8个商品辣椒品种的子叶外植体在PD培养基上形成芽丛,转移到PE培养基上伸长为具2~3个节的茎芽,茎芽在PR培养基上生根,得到了再生植株。芽分化率达100%,移栽成活率100%.外植体切除芽后回到PD培养基上的反复操作可重复诱导芽的分化,提高繁殖系数。

关键词: 辣椒, 子叶, 培养, 再生植株

Abstract: An efficient program for plant regeneration from cotyledon explants of Capsicum annuum has been developed.Using this program, plant regeneration rate with eight peppercultivars including sweet and chilli type reached 100%.This program can be used in rapid propagation of pepper hybrid as well as genetic transformation via cocultivating of leaf discs with agrobacterium.In the program the organic nutrient components have been improved and the regeneration rate, therefore, has been remarkably raised.The program is:i.shoot buds formation from pepper cotyledon explants on PD medium (20 .days).2.shoot buds elongation to shoot buds with at least two nodes (10 days).3.root formation on PR medium(20 days).4.transplanting plantlets to vormiculite pots and watering with Horland scution.5.transplanting plants on nutrient soil and culturing in green house.

Key words: Capsicum annuum, Cotyledon, Tissue culture, Plant regeneration

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引用本文

周钟信, 张宗江, 刘艳军, 江倩云, 米景九. 辣椒子叶离体培养再生植株[J]. 华北农学报, 1994, 9(2): 59-63. doi: 10.3321/j.issn:1000-7091.1994.02.012.

Zhou Zhongxin, Zhang Zongjiang, Liu Yianjun, Jiang Qianyun, Mi Jingjiu. In vitro Plant Regeneration from Cotyledon Explants of Capsicum annuum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1994, 9(2): 59-63. doi: 10.3321/j.issn:1000-7091.1994.02.012.

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