华北农学报 ›› 1993, Vol. 8 ›› Issue (S1): 83-88. doi: 10.3321/j.issn:1000-7091.1993.z1.018

所属专题: 苹果

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苹果叶片中吲哚乙酸氧化酶与过氧化物酶活性的相关性

刘魁英, 赵宗芸, 王同坤   

  1. 河北农业技术师范学院, 昌黎 066600
  • 收稿日期:1992-08-21 出版日期:1993-12-31

Correlation Between IAA Oxidase Activity and Peroxidase Activity in the Leaves of Apple Stock

Liu Kuiyin, Zhao Zongyun, Wang Tongkun   

  1. Hebei Agrotechnical Teachers’ College, Changli 066600
  • Received:1992-08-21 Published:1993-12-31

摘要: 利用PAGE分离酶谱、考马斯亮蓝R350和醋酸-联苯胺分别染色的方法分析了不同苹果砧木叶片中不同迁移率过氧化物酶带的活性和比活性,用萨尔科夫斯基试剂分析了同一叶样酶液的吲哚乙酸氧化分解的量,将吲哚乙酸氧化分解的量与不同砧木不同迁移率的过氧化物酶活性和比活性通径分析,结果表明,迁移率在0.49和0.70的两条过氧化物酶带与吲哚乙酸氧化分解的量存在着一定的相关性,但相关不显著。吲哚乙酸氧化酶的活性的迁移率在0.57附近。

关键词: 吲哚乙酸氧化酶活性, 过氧化物酶活性, 苹果, 叶片, 相关性

Abstract: The activity and the ratio activity of peroxidase with different Rf in different apple stock leaves were analysed using the PAGE and stained with Coomassie brillant blue R-250 and acetic acid-benzidine. The oxidation quantity of IAA in the same sample leaf was deter mined by use of Salkowski regent. The oxidation quantity, and the peroxidase activity and its ratio activity with different Rf in these leaves were analysed with path analyses, The re suits showed that there was certain correlation between the IAA oxidation quantity and the activity of two peroxidase bands when their Rfs were 0.49 and 0.70 respectively, but the correlation was not significant. The Rf of the IAA oxidase activity was about 0.57.

Key words: IAA oxidase activity, Peroxidase activity, Apple, Correlation

引用本文

刘魁英, 赵宗芸, 王同坤. 苹果叶片中吲哚乙酸氧化酶与过氧化物酶活性的相关性[J]. 华北农学报, 1993, 8(S1): 83-88. doi: 10.3321/j.issn:1000-7091.1993.z1.018.

Liu Kuiyin, Zhao Zongyun, Wang Tongkun. Correlation Between IAA Oxidase Activity and Peroxidase Activity in the Leaves of Apple Stock[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1993, 8(S1): 83-88. doi: 10.3321/j.issn:1000-7091.1993.z1.018.

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