摘要： 以桑树茎尖为外植体，研究了影响桑树组培快繁的关键因素。结果表明，采用低pH、软琼脂培养基加不沉滤纸托的方法，可较好地解决外植体褐变对分化的不良影响；在培养基中去掉肌醇、提高BA浓度（1.5mg/L）并配以黄化处理，可提高继代增殖率；采用“驯化+选择”的方法以食用蔗糖代替果糖，可大幅度降低培养成本；IAA与IBA配合使用使试管苗生根率达100%；移植前“增光降温”炼苗，结合在全光喷雾苗床上过渡，使移栽成活率稳定在95%以上，且可大大提高效率。

关键词: 桑树, 茎尖, 组织培养, 快速繁殖

Abstract: The key factors influencing on rapid propagation of mulberry through meristem culture were studied using its by young stem tip as explant. The results showed that subcul-turing the explant on the lower pH and soft agar medium on which there was a filter paper supporting the meristem could overcome the harmful effect of explant browning on differentiation; increasing concentration of BA (15 mg/L) on the medium without inositol could promote the germination and propagation; using the method of "domestication and selection " and taking cane sugar instead of fluctose as carbon source could decrease the culture coust greatly; using IAA and IBA cooperatively could raise the root-forming frequency to 100%; increasing light and decreasing temperature combined with whole day illumination and spraying before trasplant could make the survival rate of the transplant reach to above 95%.

Key words: Mulberry, Stem tip, Meristem culture, Rapid propagation