华北农学报 ›› 1991, Vol. 6 ›› Issue (S1): 21-26. doi: 10.3321/j.issn:1000-7091.1991.z1.004

所属专题: 小麦

• 论文 • 上一篇    下一篇

不同抗热性小麦品种热激蛋白的SDS-PAGE分析

刘植义, 沈银柱, 司智海, 张召铎, 杜晓明   

  1. 河北师范大学生物系,石家庄 050016
  • 出版日期:1991-12-31

SDS-PAGE Analysis of Heat Shock Proteins from Different Varieties of Wheat

LiuZhiyi, ShenYinzhu, SiZhihai, ZhangZhaoduo, DuXiaoming   

  1. Department of Biology, Hebei Teachers’ University, Shijiazhuang 050016
  • Published:1991-12-31

摘要: 将不同抗热性小麦品种的幼苗进行43℃高温的不同时间处理,对其水溶性蛋白质进行SDS-PAGE分析,发现热激可诱导小麦幼苗合成许多热激蛋白,同时有些正常蛋白质的合成受到抑制;水溶性蛋白质总量及有些蛋白质的含量也发生明显的变化,热激蛋白的生成量高峰出现的时期与小麦抗热性有关.由此可以检测不同小麦品种的抗热性能,为选育抗热品种提供依据.

关键词: 小麦, 热激蛋白, SDS-PAGE

Abstract: Soluble proteins from seedlings of different varieties of common wheat treated under 43℃ were analyzed with SDS-PAGE. Results indicated that heat shock could not only induce HSP but also inhibit the synthesis of some normal proteins. Both the gross of water-soluble proteins and the quantities of some proteins were found to be changed obviously. It can be inferred that the difference only in quantity-top-hour of proteins synthesized, but not in the kind-top-hours of proteins generated, may have something to do with the different resistence to heat.

Key words: Wheat, Heat shock proteins, SDS-PAGE

引用本文

刘植义, 沈银柱, 司智海, 张召铎, 杜晓明. 不同抗热性小麦品种热激蛋白的SDS-PAGE分析[J]. 华北农学报, 1991, 6(S1): 21-26. doi: 10.3321/j.issn:1000-7091.1991.z1.004.

LiuZhiyi, ShenYinzhu, SiZhihai, ZhangZhaoduo, DuXiaoming. SDS-PAGE Analysis of Heat Shock Proteins from Different Varieties of Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1991, 6(S1): 21-26. doi: 10.3321/j.issn:1000-7091.1991.z1.004.

使用本文