摘要： 利用稻瘟病的菌丝悬浮液提取稻瘟病菌毒素,以毒素浓度、毒素处理根尖的时间及不同品种进行复因子试验,结果表明.毒素处理的不同水稻品种间根冠细胞死亡率差异极显著;而不同毒素浓度处理及不同处理时间的根冠细胞死亡率差异不显著.对另外13个水稻品种在毒素抗性方面的研究结果表明,稻瘟病菌毒素对水稻根冠活细胞有较强的毒性作用,感病品种经毒素处理后细胞死亡率极显著的高抗病品种,细胞死亡率与稻穗颈瘟发病率呈极显著正相关,相关系数为0.91.这为快速鉴定水稻对稻瘟病的抗性提供了一个新方法.

关键词: 水稻, 稻瘟病菌毒素, 根冠细胞

Abstract: The pathogenic-fungi toxin of rice blast was extracted from suspension of the pathogenic-fungi hypha. The experiments of multiple factors (concentration of toxin, the duration of treatment of root-cap with the toxin, the rice variety) were done. The results indicated that there was very significant difference between various rice varieties treated with toxin in the mortality of root-cap cells, and the differences neither between the toxin concentrations and nor between the durations of treatments with the toxin were significant. In addition, an observation on the resistance of 13 rice varieties to the toxin showed that the pathogenic-fungi toxin of rice blast exerted stronger toxicity on living root-cap cells of rice, the cell mortality of susceptible varieties treated with toxin was remarkably higher than that of resistant varieties, cell mortality significantly correlated with morbility of neck rot disease of rice, and the correlation coefficient was 0.91. This study would afford a new way to identify quickly rice resistance to rice blast.

Key words: Rice, Pathogenic-fungi toxin of rice blast, Root cap cellll