Differential Expression Analysis of PSY1 Gene in Peppers with Different Ripe Fruit Colors

doi:10.7668/hbnxb.20194772

Abstract

Abstract:

In order to study the mechanism of PSY1 gene in different ripe fruit colors of peppers, Y15016,Y15016-2,SP01,SP02 and Z1 were used as materials,and the functional properties of PSY protein and the expression of PSY1 gene in different fruit color materials of pepper were studied and analyzed by combining some bioinformatics methods.The results showed that the full-length PSY1 gene could be cloned in all five pepper varieties,and there was no difference in sequence.Gene structure analysis showed that the PSY1 gene contained six exons and five introns with a total length of 2 844 bp,and its CDS contained 1 260 bp and encoded 419 amino acids.Sequence alignment and phylogenetic tree analysis showed that the PSY protein of pepper was the closest to the homologous PSY protein of tomato and tobacco of the same family.The results of qRT-PCR analysis showed that the expression level of PSY1 gene in the five materials was lowest in the root tissue and highest in the leaf tissue.Besides,the PSY1 gene was expressed in all the tissues of these materials.The expression level of orange mutant Y15016-2 was higher than that of wild-type Y15016,while that of yellow mutant SP02 was significantly lower than that of wild-type SP01.At different stages of fruit development,the expression of PSY1 gene increased with the development of fruits,except for the decrease in stage Ⅲ,and reached the maximum value at the maturity stage(Ⅳ—Ⅴ)of different fruit color materials.The results of PSY1 gene promoter analysis showed that there was no difference in the sequences in the test materials.The results suggest that the differential expression of PSY1 gene may play an important role in the formation of peppers with different fruit colors.

Key words: Pepper, Phytoene synthase, Carotenoid metabolic pathway, Gene cloning, Gene expression

TAO Gongchen, MA Yujie, WEN Junqin, WANG Yayi, LI Quanhui. Differential Expression Analysis of PSY1 Gene in Peppers with Different Ripe Fruit Colors[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 15-24. doi: 10.7668/hbnxb.20194772.

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Figures/Tables 12

Fig.1 Experimental material A.Y15016;B.Y15016-2;C.SP01;D.SP02;E.Z1。

Fig.2 Cloning and gene structure chart of PSY1 gene in Capsicum annuum L. A.Agarose gel electrophoresis of PSY1 gene cloning in Capsicum annuum L.;B.The gene structure prediction of PSY1 in Capsicum annuum L..

Fig.3 CDS full length and prediction amino acid sequence of PSY1 gene in Capsicum annuum L.

Fig.4 Pepper PSY protein signal peptide and transmembrane domain prediction A.Protein signal peptide prediction of pepper PSY protein;B.Transmembrane domain prediction of pepper PSY protein.

Fig.5 PSY amino acid sequence hydrophilicity and hydrophobicity analysis in Capsicum annuum L.

Fig.6 Prediction of PSY protein secondary and tertiary structure in Capsicum annuum L. A.Prediction of PSY protein secondary structure in Capsicum annuum L.;B.Prediction of PSY protein tertiary structure in Capsicum annuum L..

Fig.7 Prediction of PSY amino acid sequence conservative domain in pepper

Fig.8 PSY protein multiple comparison of amino acid sequence between Capsicum annuum L. and other plants

Fig.9 PSY proteins amino acid sequence phylogenetic tree of Capsicum annuum L. and other plants

Fig.10 PSY1 genes relative expression levels in different tissues of full-blown pepper during five varieties Different lowercases represent significant differences in PSY1 gene expression between tissue development(P<0.05);different uppercases represent significant differences in PSY1 gene expression between different varieties(P<0.05).

Fig.11 PSY1 genes relative expression levels in different periods of pepper fruit in five varieties Different lowercases represent significant differences in PSY1 gene expression between fruit development(P<0.05);different uppercases represent significant differences in PSY1 gene expression between different varieties(P<0.05).

Fig.12 PSY1 gene promoter analysis in pepper A.Alignment of PSY1 gene promoter sequences in Y15016,Y15016-2,SP01,SP02,Z1 and reference genome CM334;B.PSY1 gene promoter cis-acting element prediction.

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