Abstract:

In order to comprehensively and systematically explore the small G protein ROP of Rosaceae, it provides candidate genes for disease resistance breeding of Rosaceae at the molecular level. Phylogenetic relationship analysis, ROP protein genome-wide screening, Rho functional domain analysis, protein cluster analysis and physicochemical property analysis used NCBI, iTOLv6, GDR, SMART, MEGA 11.0 and Maximum-likelihood algorithm1, Protparam and Expasy databases and bioinformatics online software, respectively. Eleven species of Rosaceae were clustered into 6 subclades; 80 ROP proteins were screened in 11 species of Rosaceae; 80 Rosaceae ROP proteins and 11 Arabidopsis ROP proteins were aggregated into 6 evolutionary branches; the protein length, isoelectric point, molecular weight, fat index and instability index were 143—215 aa, 5.59—9.63, 15.735 99—23.878 59 ku,85.28—94.95 and 27.96—57.14, respectively. 80 Rosaceae ROP proteins were the most abundant in Fragaria ananassa and the least in Prunus avium; Rosaceae ROP proteins were distributed in branches Ⅳ, Ⅴ and Ⅵ, Arabidopsis ROP proteins were distributed in branches Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ, that is, branch Ⅵ was a Rosaceae-specific branch, and branches Ⅰ, Ⅱ and Ⅲ were Arabidopsis-specific branches.

Key words: Rosaceae, Small G protein ROP, Genome-wide screening, Rho functional domain, Phylogenetic relationship, Physicochemical characteristics of protein