Pan-genomic Identification and Expression Pattern Analysis of the OPR Gene Family in Maize

doi:10.7668/hbnxb.20194096

Abstract

Abstract:

12-oxo-phytodienoic(OPR) acid reductase is a flavin mononucleotide-dependent oxidoreductase,a key enzyme for the synthesis of jasmonic acid,which is important for plant growth, development and defence regulation.In order to study the disease resistance effect of OPR gene in maize, bioinformatics methods were used to identify OPR family members in 31 different maize inbred lines and the expression pattern after infestation by S. turcica was analyzed.The results showed that eight OPR genes were identified in maize line B73 and they were unevenly distributed on seven chromosomes.In addition,these expressed proteins of OPR genes were rich in acidic amino acids.Further analysis revealed that the maize OPR family contained only one structural domain,Oxidored_FMN,and all OPR members contained 10 protein conserved motifs identified.Phylogenetic relationship analysis of OPR family members from maize,wheat,rice and Arabidopsis using MEGA software revealed significant differences in the evolutionary relationships of OPR gene families in these plants.The OPR proteins of maize and those of rice were the closest in evolution.Homology analysis using OrthoFinder software revealed that the maize OPR gene family was highly conserved,with all OPR genes being core genes,but with slightly different functions.According to the previous RNA-seq data of Hebei key Laboratory of Plant Physiology and Molecular Pathology,we analyzed the expression pattern of OPR genes of maize B73 in response to S.turcica infection and verified the gene expression pattern of OPR genes through qRT-PCR.It was found that there were three different expression patterns in the process of S.turcica infection.This study systematically identified the maize pan-genomic OPR family genes and their expression patterns in response to S.turcica infection.

Key words: Maize, OPR family, Phylogenetic, Pan-genome, Expression pattern

LI Wenfei, LI Hongxia, LIU Yuwei, GONG Xiaodong, WEI Shuzhen, GU Shouqin. Pan-genomic Identification and Expression Pattern Analysis of the OPR Gene Family in Maize[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(5): 51-59. doi: 10.7668/hbnxb.20194096.

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Figures/Tables 11

References 23

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基因序号 Gene ID	氨基酸数量/个 Number of amino acids	分子质量/ku Molecular weight	理论等电点 Theoretical isoelectric point(pI)
Zm00001eb373030	373	41.592 98	6.47
Zm00001eb373050	376	41.901 20	5.85
Zm00001eb279210	377	41.616 81	5.33
Zm00001eb131870	372	41.393 61	5.37
Zm00001eb040310	399	44.103 77	8.53
Zm00001eb082050	391	43.489 38	6.76
Zm00001eb177330	399	44.220 88	7.15
Zm00001eb356160	383	42.216 62	5.54

基因序号 Gene ID	氨基酸数量/个 Number of amino acids	分子质量/ku Molecular weight	理论等电点 Theoretical isoelectric point(pI)
Zm00001eb373030	373	41.592 98	6.47
Zm00001eb373050	376	41.901 20	5.85
Zm00001eb279210	377	41.616 81	5.33
Zm00001eb131870	372	41.393 61	5.37
Zm00001eb040310	399	44.103 77	8.53
Zm00001eb082050	391	43.489 38	6.76
Zm00001eb177330	399	44.220 88	7.15
Zm00001eb356160	383	42.216 62	5.54

玉米品系 Maize strains	OPR蛋白个数 Number of OPR proteins	玉米品系 Maize strains	OPR蛋白个数 Number of OPR proteins
B73	11	Ky21	12
B97	16	M37W	11
CML52	14	M162W	12
CML69	12	Mo17	11
CML103	10	Mo18W	18
CML228	10	Ms71	11
CML247	13	NC350	16
CML277	9	NC358	13
CML322	15	Oh7B	11
CML333	15	Oh43	13
EP1	8	P93	16
F7	8	PH207	8
HP301	14	Tx303	12
Il14H	12	Tzi8	14
Ki3	9	W22	10
Ki11	11

玉米品系 Maize strains	OPR蛋白个数 Number of OPR proteins	玉米品系 Maize strains	OPR蛋白个数 Number of OPR proteins
B73	11	Ky21	12
B97	16	M37W	11
CML52	14	M162W	12
CML69	12	Mo17	11
CML103	10	Mo18W	18
CML228	10	Ms71	11
CML247	13	NC350	16
CML277	9	NC358	13
CML322	15	Oh7B	11
CML333	15	Oh43	13
EP1	8	P93	16
F7	8	PH207	8
HP301	14	Tx303	12
Il14H	12	Tzi8	14
Ki3	9	W22	10
Ki11	11

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