Genome-wide Association Study of Spike-related Traits of Bread Wheat in North Huanghuai River Valley Wheat Region

doi:10.7668/hbnxb.20190900

Abstract

Abstract: In order to identify the significant association loci and the favorable alleles that are responsible for spike-related traits,genome-wide association study (GWAS) was conducted in this research using an association panel consisted of 132 elite wheat varieties and their advanced lines mainly from the North Huanghuai River Valley Wheat Region. Wheat 55K SNP array was used to perform marker-trait association analysis. Four spike-related traits were evaluated at 8 environments,involving spike length,total spikelet number per spike,kernel number per spike and spike compactness. A total of 121 significant association SNP loci were identified,containing 38 sites significantly associated with spike length,15 sites significantly associated with total spikelet number per spike,47 sites significantly associated with spike compactness,and 21 sites significantly associated with kernel number per spike. There were 8 SNPs detected in multiple environments,including AX-108730544-1D, AX-94755570-2A, AX-111703851-6D, AX-111235532-2A, AX-110926142-2A, AX-110378404-5A, AX-108730544-1D and AX-110963299-1D. Geographical distribution analysis indicated that frequencies of the favorable alleles at the 8 significant association loci were divergent among theprovinces in the Huanghuai River Valley Wheat Region. The pyramiding effect analysis showed that the phenotypic value of spike length,total spikelet number per spike and spike compactness were increased with incorporation of the favorable alleles. These results provide a fundamental basis for gene mining and marker-assisted selection breeding of wheat panicle traits.

Key words: Bread wheat, Huanghuai River Valley Wheat Region, Spike-related traits, GWAS, Marker-assisted selection breeding

CLC Number:

S512.03

ZHANG Shuai, ZHANG Xilan, ZHANG Na, ZHAO Minghui, QIAO Wenchen, SUN Lijing, LI Hui, FU Xiaoyi, HE Mingqi, JI Jun, LI Junming. Genome-wide Association Study of Spike-related Traits of Bread Wheat in North Huanghuai River Valley Wheat Region[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 31-39. doi: 10.7668/hbnxb.20190900.

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