Proteomics Analysis of Rice Root under Low Temperature Stress at Seedling Stage

doi:10.7668/hbnxb.20190090

Abstract

Abstract: In order to reveal the molecular mechanism of response to low temperature stress at seedling stage, wild rice Dongxiang with cold tolerance and cultivar Zhongjiazao 17 with cold sensitivity were taken as the tested materials, and their root activity, peroxidase, superoxide dismutase activity and malondialdehyde content were measured under low temperature condition of 5℃ for 0, 1, 2,3 days, respectively. The differential proteomics of seedling roots in the two varieties treated at 5℃ for 3 d were analyzed by iTRAQ. The results indicated that the physiological indexes had obvious differences between the two rice materials under the treatment of 5℃ for 3 d. The differential proteomics analysis showed that a total of 167 differentially expressed proteins were identified in Dongxiang wild rice, of which 61 were up-regulated and 106 down-regulated, and a total of 261 differentially expressed proteins were identified in cultivar Zhongjiazao 17, of which 122 were up-regulated and 139 down-regulated. GO analysis indicated that these differentially expressed proteins mainly participated in metabolic process, celluar process, and single-organism process related to cell and cell part, playing the binding and catalytic effects. KEGG analysis showed that these differentially expressed proteins mainly related to metabolic pathways, biosynthesis of secondary metabolites and carbon metabolism. Through further analysis of 29 proteins expressed differentially in both Dongxiang wild rice and cultivar Zhongjiazao 17, the expression dynamics of some proteins in root was obtained after they were damaged by low temperature. The identification of differentially expressed proteins would be helpful to discover the cold tolerance genes and explain the cold tolerance mechanism of rice.

Key words: Rice, Low temperature stress, Proteomics, Seedling stage, Root

CLC Number:

S332.5

TANG Xiuying, WANG Huimin, LONG Qizhang, HUANG Yonglan, LU Ming, WAN Jianlin. Proteomics Analysis of Rice Root under Low Temperature Stress at Seedling Stage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 82-88. doi: 10.7668/hbnxb.20190090.

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