Abstract: To obtain the novel menbers of Pinellia pedatisecta lectin gene family,according to Arisaema lectin gene sequence reported in GenBank,three agglutinin genes were amplified by PCR with designed primers from DNA extracted from leaves of Pinellia pedatisecta,temporarily named PPA15, PPA324, PPA533.The full length of PPA15, PPA324 and PPA533 were 729,774,777 bp,respectively,encoded 243,258,259 amino acids.Bioinformatics analysis showed that the PPAs were high homology with Araceae pinellia genes of GenBank,moreover,they all had mannose unique binding sites in monocotyledonous plants,suggesting that they might belong to the same gene family.The genes were characterized by signal peptide,which consisted of 25 amino acid residues at the N terminal.They had typical transmembrane domain and were presumed to be localized in the inner membrane structure.Constructed the prokaryotic expression vectors pET-28b (+)-PPAs,the recombinant proteins expressed in Escherichia coli BL21 (DE3),moreover,results of SDS-PAGE showed that the recombinant protein had molecular weight about 28.0 ku,which was consistent with the expectation.The results laid the foundation for the further study of Pinellia pedatisecta lectin family proteins function.

Key words: Pinellia pedatisecta, Lectin, Vector construction, Prokaryotic expression

CLC Number: 

• Q78

• S567.03