Development of Immune Tissue and Correlation Analysis of TLRs Expression in Gaoyou Duck

doi:10.7668/hbnxb.2018.01.018

Abstract

Abstract: TLRs play an important role in natural immunity.Development of body, thymus, spleen, bursa fabriciu were measured, and the expression levels of TLR1, TLR2, TLR4, TLR5 (TLR1/2/4/5)were detected in these immune tissues by fluorogenic qRT-PCR in Gaoyou duck. The results revealed that the expressions of TLR1/2/4/5 mRNA were detected and showed different pattern in thymus, spleen, bursa fabricius during production period (8 weeks). Expression of TLR1/2/4/5 in thymus at 1 week was higher than those in other weeks, and the higher expressions of TLR1/2/5 mRNA in spleen were showed at 8 weeks, expression of TLR1/5 in bursa fabricius was higher at 8 weeks. Analysis of correlation revealed that there were high index between body weight and immune tissue weight, while the index of expression of TLR1/2/4/5 mRNA and weights of immune tissue were less than the index of expression of TLR1/2/4/5 mRNA and body weight. The expression of TLR1/2/4/5 mRNA in thymus exhibited relatively high correlated index with each other. In conclude, these results can reveal expression pattern of TLR1/2/4/5 during the process of immune organ development.

Key words: Gaoyou duck, Immune tissue, Development, Relative expression of TLRs mRNA

CLC Number:

S834.03

ZHU Chunhong, LIU Hongxiang, TAO Zhiyun, XU Wenjuan, SONG Weitao, ZHANG Shuangjie, LI Huifang. Development of Immune Tissue and Correlation Analysis of TLRs Expression in Gaoyou Duck[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(1): 115-120. doi: 10.7668/hbnxb.2018.01.018.

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