Cloning and Activity Analysis of Cold Induced GmERF9P Promoter from Soybean

doi:10.7668/hbnxb.2017.05.003

Abstract

Abstract: Promoters play a key role in the regulation of gene expression. The objective of this study is to isolate and characterize the GmERF9 promoter (GmERF9P) from soybean. The GmERF9P was amplified with PCR from the genome DNA of soybean,which had a length of 1 885 bp. Sequence analysis indicated that GmERF9P contained a number of stress-related elements. The GmERF9P was cloned into plant expression vector of pCAMBIA1301 and transformed into tobacco NC89. The regenerated plants were analyzed by PCR and showed that the promoter fragments were integrated into six genomes of tobacco plants. Histochemical staining of GUS activity and Real-time fluorescent quantitative PCR of the transgenic plants showed that the expression of GUS gene was increased under cold treatment for 2 h,which proved that GmERF9P was an efficient cold-inducible promoter.

Key words: Soybean, GmERF9P, Promoter, Cold induced, Transgenic tobacco

CLC Number:

Q78
S565.03

ZHAI Ying, ZHANG Jun, REN Weiwei, ZHANG Chuang, ZHAO Yan, ZHANG Meijuan. Cloning and Activity Analysis of Cold Induced GmERF9P Promoter from Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 13-18. doi: 10.7668/hbnxb.2017.05.003.

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http://www.hbnxb.net/EN/Y2017/V32/I5/13

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