Optimization of Inter-simple Sequence Repeat PCR Reaction System of Phyllosticta citricarpa

doi:10.7668/hbnxb.2016.01.020

Abstract

Abstract: In order to obtain the optimization of ISSR-PCR reaction system of Phyllosticta citricarpa (McAlpine) van der Aa,the concentrations of five factors (Mg²⁺,dNTP,primers, Taq DNA polymerase,template) were respectively optimized by single factor test.The result showed that the optimized reaction system consisted of 1.5 mmol/L Mg²⁺,0.15 mmol/L dNTP,0.3 μmol/L primers,25 ng template DNA,0.5 U Taq DNA polymerase.The optimized ISSR-PCR reaction system was stable and credible by the amplification of 21 strains of P.citricarpa.The study would provide the basis for the genetic analysis of P.citricarpa.

Key words: Phyllosticta citricarpa, Inter-simple sequence repeat, Single factor test, Citrus black spot

CLC Number:

S435.79

WANG Xinghong, LI Hongye. Optimization of Inter-simple Sequence Repeat PCR Reaction System of Phyllosticta citricarpa[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 123-127. doi: 10.7668/hbnxb.2016.01.020.

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