Sequence Analysis of Whole Genome of Porcine Reproductive and Respiratory Syndrome Virus Shanxi-6 Strain

doi:10.7668/hbnxb.2014.02.011

Abstract

Abstract: The fifteen gene fragments of Shanxi-6 strain of porcine reproductive and respiratory syndrome were amplified respectively by RT-PCR, cloned into pMD18-T vector and sequenced. The genome of Shanxi-6 strain in length was 15 320 bp excluding PloyA tail, containing 9 open reading frames (ORFs), the 5'UTR in 189 bp and the 3'UTR in 150 bp. The complete genomic analysis showed that Shanxi-6 strain shared 54. 35% of nucleotide ho- mology with European genotype (LV) and shared 88. 80% homology with American genotype (VR-2332) . Shanxi- 6 strain has correlative genetic relationship and shared 97. 44%-97. 74% homology with highly pathogenic PRRSV (JXA1, HUN4, HuN, HUB1) . 30 amino acids deletion was exhibited in Nsp2 protein of Shanxi-6 strain.

Key words: PRRSV, Complete genome, Sequence analysis

CLC Number:

HAN Yi-chao, LIU Wen-jun, YAO Jing-ming, WU Xin, WANG Juan-ping, WU Shou-yan, CHEN Jian-bo, MENG Fan, GUO Hui-hui, YANG Li-hua, LEI Yu-ping, FAN Zhen-hua. Sequence Analysis of Whole Genome of Porcine Reproductive and Respiratory Syndrome Virus Shanxi-6 Strain[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 62-65. doi: 10.7668/hbnxb.2014.02.011.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.02.011

http://www.hbnxb.net/EN/Y2014/V29/I2/62

References

[1] Hall W V. Porcine reproductive and respiratory syndrome(PRRS) virus a significant disease of pigs[J]. Aust VetJ,2005,83(5):260-261.
[2] Barbara E Straw, Jeffery J Zimmerman, Sylvie D'Allaire,等. 猪病学[M]. 9 版．赵德明, 张仲秋, 沈建忠, 等译.北京: 中国农业大学出版社,2008:423-463.
[3] Murtaugh M P, Elam M R, Kakach L T. Comparison of thestructural protein coding sequences of the VR-2332 andLelystad virus strains of the PRRS virus[J]. Arch Virol,1995,140(8):1451-1460.
[4] Dea S, Gagnon C A, Mardassi H, et al. Current knowledgeon the structural proteins of porcine reproductive and re-spiratory syndrome (PRRS ) virus: comparison of theNorth American and European isolates [J]. Arch Virol,2000,145(4):659-688.
[5] 郭宝清,陈章水,刘兴文,等. 从疑似 PRRS 流产胎儿分离PRRSV 的研究[J]. 中国畜禽传染病,1996(2):1 -4.
[6] 童光志, 周艳君, 郝晓芳, 等. 高致病性猪繁殖与呼吸综合征病毒的分离鉴定及其分子流行病学分析[J].中国预防兽医学报,2007,29(5):323-326.
[7] Tian K, Yu X, Zhao T, et al. Emergence of fatal PRRSVvariants: unparalleled outbreaks of atypical PRRS in Chi-na and molecular dissection of the unique hallmark[J].PloS One,2007,2(6):526.
[8] Ansari I H, Kwon B, Osorio F A, et al. Influence of Nlinked glycosylation of porcine reproductive and respirato-rysyndrome virus GP5 on virus infectivity,antigenicity,andability to induce neutralizing antibodies [J]. J Virol,2006,80(8):3994-4004.
[9] Gonin P,Pirzadeh B,Gagnon C A,et al. Seroneutralization ofporcine reproductive and respiratory syndrome virus corre-lates with antibody response to the GP5 major envelope gly-coprotein[J]. J Vet Diagn Invest,1999,11(1):20 -26.
[10] 周宏专, 徐福洲, 王金洛, 等. 猪繁殖与呼吸综合征病毒 ORF5 基因不同编码区的表达和反应原性鉴定[J]. 华北农学报,2007,22(3):12-15.
[11] Ostrowski M, Galeota J A, Jar A M, et al. Identification ofneutralizing and nonneutralizing epitopes in the porcinereproductive and respiratory syndrome virus GP5 ectodo-main[J]. J Virol,2002,76(9):4241-4250.

[1]	MA Jun, LIAN Kaiqi, SHAN Jinyan, LIU Yuling, LI Xiaolong, ZHAO Liming, LI Xuejun, PENG Renhai. *Cloning and Expression Analysis of bmp6 Gene in Carassius auratus* var.Qihe** [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(2): 218-229.
[2]	PENG Haifen, WANG Guojun, DUAN Xinhui, LIU Lixian, YANG Shasha, ZHANG Yifang, LI Wengui. *Analysis of the Genetic Diversity of Bovine ephemeral fever virus* G Gene in Yunnan Province of China** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(S1): 281-288.
[3]	HUA Mingyan, SONG Lanfang, CUI Shaojie, SUN Haibo, JIN Fengmei. Molecular Detection and Genome Sequence Analysis of Tomato brown rugose fruit virus [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 179-186.
[4]	WANG Xinmiao, ZHAO Mengliang, SHAO Dengkui, GA Sang, REN Yanjing. *Cloning and Expression Analysis of MYB62* Transcription Factors from Kohlrabi** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(2): 62-70.
[5]	LI Chenyu, ZUMUREMU Tolson, LI Xiaorong, YANG Yang, LI Bo, YU Yuehua. *Cloning and Prokaryotic Expression of the GhMYB42* Gene in Cotton** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 48-54.
[6]	ZHANG Xi, QIAN Yuyuan, WANG Guang'en, LIU Yi, QUAN Yuewei, CUI Shufang, MI Huanfang, LI Junlan. Cloning and Expression Analysis of a Cotton Pigment Gland Formation Related Gene GhERF105-like [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(6): 34-41.
[7]	XIAO Shikui, LI Fang, ZHANG Wenting, LÜ Shufang, SHI Guoan, WU Jiang, FAN Bingyou. *Cloning and Prokaryotic Expression of ACS* Gene from Paeonia lactiflora Pall.** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 36-44.
[8]	LIAN Kaiqi, JI Shuang, ZHOU Lingling, SHI Zhiqi, WANG Fei, ZHANG Yuanchen. Cloning and Prokaryotic Expression of a clip-type Serine Protease Gene from Mythimna separate [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 195-201.
[9]	JIN Fengmei, XUE Jun, SUN Haibo, HAO Zhiyu. *Molecular Detection and Partial Genomic Sequence Analysis of Tomato mosaic virus* in Tianjin Based on Small RNA Sequencing** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 176-183.
[10]	AERZUGULI Maimaitituerxun, ZHANG Zijun, ZHANG Weihong. *Complete Mitochondrial Genome Sequence of Oxyloma wujiaquensis* Li, Guo & Zhang, 2017(Gastropoda：Succineidae)** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 230-238.
[11]	HU Yueqing, WANG Ruozhong, HUANG Zhigang, XIAO Langtao. *Cloning and Prokaryotic Expression of Transcription Factor StWRKY57* Gene in Potato** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 10-17.
[12]	ZHANG Xi, QIAN Yuyuan, LIU Yi, WANG Guang, SONG Shijia, LI Xiaofei, MI Huanfang, CUI Shufang, LI Junlan. Cloning and Expression Analysis of a Cotton Pigment Gland Formation Related Gene GhNAC201 [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 29-35.
[13]	ZHEN Junbo, LIU Di, SONG Shijia, LIU Linlin, CAI Xiao, ZHANG Xi, LI Haishan, CHI Ji. Cloning and Expression Analysis of Two Late Embryogenesis Abundant(LEA) Proteins from Upland Cotton [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 33-38.
[14]	LIU Yufeng, ZHU Tianhui, LIU Yinggao, QIAO Tianmin, LI Shujiang, LONG Xumei, HAN Shan. *Cloning, Sequence Analysis and Prokaryotic Expression of CmWRKY* from Castanea mollissima BL** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 37-45.
[15]	SONG Jian, XUE Jun, JIN Fengmei, SUN Haibo, ZHANG Yue, WANG Shu, FAN Huan, ZHOU Xiangming, CHEN Rui. Partial Virus Gene Sequence and Real-time Quantitative PCR Analysis of Tomato after Mixed Infection of ToCV and TYLCV [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 95-102.

Please choose a citation manager

Content to export