Reference Gene Expression in Mammary Tissue of Periparturient Cows Is Affected by Dietary Forage to Concentrate Ratio

doi:10.3969/j.issn.1000-7091.2013.04.014

Abstract

Abstract: Selection of appropriate reference genes for normalization of quantitative PCR data for genes of inter- est is critical for interpretation of results．Seven candidate reference genes including GAPDH，ACTB，18S rRNA， RPS9，RPS15，UXT and MYC were selected in this research．Cows were fed different forage to concentrate(F: C) ra- tio dietary and mammary biopsies were taken at 16 day postpartum．The mRNA expression levels of seven candidate reference genes in mammary tissue were detected by qRT- PCR．Gene copy number was determined using absolute quantification，and then the expression stability of these genes were analysed by geNorm software．These results sug- gested that UXT and RPS9 showed less variability between the cows and were unaffected by dietary F: C ratio and， therefore，could be used to normalize mRNA levels of mammary in periparturient cows．

Key words: Periparturient cows, Mammary gland, Reference gene, qRT- PCR, geNorm software

CLC Number:

QIN Tong, WANG Hao-yu, HAO Hai-sheng, WANG Dong, DU Wei-hua, ZHU Hua-bin. Reference Gene Expression in Mammary Tissue of Periparturient Cows Is Affected by Dietary Forage to Concentrate Ratio[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 75-78. doi: 10.3969/j.issn.1000-7091.2013.04.014.

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