Abstract: Hebei Yali pear as the test materials，through the determination of fruit browning index and the vari- ation trend of LOX isozymes of pulp，slow and rapid cooling treatment were used for studying browning effect and LOX isozymes activity of different harvest maturity pulp．The results showed that a serious degree of browning was as follows: late harvest fruit ＞ early fruit picking ＞ middle fruit picking，rapid cooling ＞ slow cooling．Therefore， the best storage methods was that mining maturity harvest pear(mid- September) using the slow cooling treatment． On the other hand，was determined with non- denatured PAGE．There were 9 LOX isozyme belts in pulp of Yali．And the rapid and slow cooling for different maturity of Yali LOX isozymes had a certain effect．The expression of LOX isozymes for rapid cooling was higher than that for slow cooling．The order of LOX isozymes expression for different maturity of Yali was late fruit ＞ middle fruit ＞ early fruit．This result was consistented with the determination of fruit browning index．It implicated that LOX enzyme isozymes had a close relationship with pears pulp browning．

Key words: Yali pear, Pulp, Browning index, LOX isoenzymes, Non- denatured PAGE

CLC Number: 

• S661.2