Isolation， Identification and Molecular Characterization for Fgene of Newcastle Disease Virus Isolated in Shandong

doi:10.3969/j.issn.1000-7091.2013.02.041

Abstract

Abstract: Four Newcastle Disease Virus isolates named SD01-SD04 were collected from chicken in Shandong in 2011. The four strains were purified by plaque assay can form typical cytopathic. The Fgenes of the isolates were cloned and sequenced to study its molecular characterization and hereditary variation. The deduced amino acid se-quence of Fgene showed that these viruses shared a velogenic motif 112RRQKRF 117at the cleavage site which were coincident with biological index. Phylogenetic analysis showed that SD01-SD04 and the viruses derived from chicken andgoose in China belonged togenotype Ⅶ. For Fgene of the isolates,the homologies of the deduced amino acid se-quences of SD01-SD04 with the same time isolates in China were higher compared with traditional trains. There was a closergenetic relationship between the four isolates and epidemic strains in China than foreign epidemic strains.

Key words: Newcastle disease virus, Fgene, Molecular characterization

CLC Number:

S432. 4

YANG Jin-xing, ZHU Rui-liang. Isolation， Identification and Molecular Characterization for Fgene of Newcastle Disease Virus Isolated in Shandong[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 231-234. doi: 10.3969/j.issn.1000-7091.2013.02.041.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2013.02.041

http://www.hbnxb.net/EN/Y2013/V28/I2/231

References

[1] 吴洪俊,刘文斌,彭永刚. 新城疫的流行特点[J]. 中国兽医杂志,2004,40(1):34-37.
[2] 王华,程太平. 大黄乙醇提取物的鸡体内抗新城疫病毒试验[J].天津农业科学,2012,18(2):23-26.
[3] 银梅,赵坤,陈桂香,等. 鸡新疫病毒新乡株MD-XX10的分离与鉴定[J]. 河南农业科学,2011,40(2): 142-145.
[4] Qin Z M,Tan L T,Xu H Y,et al. Pathotypical character-ization and molecular epidemiology of Newcastle Disease Virus isolates from different hosts in China from 1996 to 2005[J]. J Clin Microbiol,2008,46(2):601-611.
[5] Yuan X Y，Wang Y L,Yang J X,et al.genetic and bio-logical characterizations of a Newcastle disease virus from swine in china[J]. Virology Journal,2012,9:129.
[6] Liu X F,Wan H Q,Ni X X,et al. Pathotypical andgeno-typical characterization of strains of Newcastle disease vi-rus isolated from outbreaks in chicken andgoose in some regions of China during 1985-2001[J]. Archives of Vi-rology,2003,148(7):1387-1403.
[7] Mohammed M H,Hair-Bejo M,Omar A R,et al. Molecu-lar changes of the fusion proteingene of chicken mesen-chymal stem cells – adapted velogenic[J]. Journal of Advanced Biomedical ＆ Pathobiology Research,2012,2 (5):1-59.
[8] 李红丽,詹丽娥,张伟业,等. 麻鸡新城疫病毒SX-1株F和HN基因在真核细胞中共表达研究[J]. 华北农学报,2011,26(3):47-50.
[9] 张丽青,王爱华,张秀珊,等. 新城疫病毒河北分离株F基因的克隆及序列分析[J]. 华北农学报,2008,23 (4):19-22.
[10] 符芳,姜北宇,张莉,等. 鸡新城疫病毒La Sota株F基因的克隆及原核表达[J]. 华北农学报,2006,21 (3):117-120.
[11] Qin Z M,Sun L,Ma B C,et al. Fgene recombination be-tweengenotypeⅡ and Ⅶ Newcastle disease virus[J]. Virus Research,2008,13(2):299-303.
[12] McGinnes L W,Gravel K A,Finberg R W,et al. Assem-ble and immunological properties of Newcastle Disease virus-like particles containing the respiratory Syncytial Virus F andg Proteins[J]. Journal of Virology,2011,85 (1):366-377.
[13] Jahangir A,Ruenphet S,Ueda S,et al. Avian influenza and Newcastle disease viruses from northern pintail in Japan:Isolation，characterization and inter-annual com-parisons during 2006-2008[J]. Virus Research,2009,143(1):44-52.
[14] 刘华雷,蒋小刚,张维,等.一株 ClassI新城疫病毒中国分离株分子特性的研究[J]. 中国动物检疫,2008,25(8):30-33.
[15] 刘华雷,王永坤,中国部分地区新城疫病毒的分子流行病学研究[J]. 扬州大学学报:自然科学版,2001,4 (1):35-40.
[16] 吴艳涛,倪雪霞,万洪全,等.我国部分地区不同动物来源新城疫病毒的分子流行病学研究[J]. 病毒学报,2002,18(3):264-269.
[17] Liu X,Wan H,Ni X,et al. Pathotypical andgenotypical characterization of strains of newcastle disease virus iso-lated from outbreaks in chicken andgoose flocks in some regions of China during 1985-2001[J]. Archives of Vi-rology,2003,148(7):1387-1403.

Please choose a citation manager

Content to export