Epidemiology of Marek's Disease in Commercial Layer Chickens in Henan Province

doi:10.3969/j.issn.1000-7091.2012.06.032

Abstract

Abstract: In 2011-2012， the tumorigenic and immunosuppresive disease were broken out in the commercial layer chickens in large areas of Henan province． The clinical symptoms and macroscopic pathological inspections suggested that it might be a case of Marek's disease ( MD). In order to identity the cause of the disease， three pairs of primers specific to the MDV meq，gB and pp38genes were designed according to the genomic sequence of MDV strain GA． Meanwhile，polymerase chain reaction ( PCR) was carried out on liver and spleen samples of those suspected cases． The results showed that three different sizes of PCR products of MDV-specific genes were successfully amplified from the randomly tested five cases collected in each region． It indicated that MDV was widely spread in Henan Provice and led to the outbreak of MD in the commercial layer chickens． The study provided useful information for the rapid diagnosis and efficient control of MD in the future．

Key words: Marek's disease, Epidemiology, MDV, PCR

CLC Number:

S831.7

YU Le-le, TENG Man1, LUO Jun, WANG Xin-wei, SU Jing-wei, CHAI Shu-jun, WANG Ai-ping, ZHANG Gai-ping. Epidemiology of Marek's Disease in Commercial Layer Chickens in Henan Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 163-166. doi: 10.3969/j.issn.1000-7091.2012.06.032.

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