SeNHX1 Protein Localization and Its Function of Na~＋-detoxification

doi:10.3969/j.issn.1000-7091.2012.05.002

Abstract

Abstract: Yeast expression vector pYES2-SeNHX1,pYES2-GFP,pYES2-SeNHX1-GFP were introduced into the nhx1 mutant strain 296H.The protein localization of SeNHX1 was observed by confocal laser scanning microscope and the function of Na＋-detoxification was studied by growth of 296H with different expression vector in medium containing 0.5 mol/L NaCl.The results showed that green fluorescence dispersed in the whole cell in 296H(pYES2-GFP),but only appeared on the vacuolar membrane and shaped a cyclic structure in 296H(pYES2-SeNHX1-GFP).So SeNHX1 was guessed to be localized on the vacuolar membrane.The strains of 296H(pYES2-GFP) and 296H were inhibited for growth when cultured in medium with NaCl,where the strains with SeNHX1 gene grew better.The finding suggested SeNHX1 gene could complement the function of nhx1 mutant.Therefore,on the base of amino acid sequence analysis and hydrophobicity analysis,SeNHX1 was confirmed as vacuolar Na＋ /H＋ antiporters,which conferred to the salt tolerance of plants.

Key words: SeNHX1 gene, Yeast, Expression vector, GFP green fluorescence, Salt-tolerance, SeNHX1 localization.

CLC Number:

S184

YANG Xiao-ling, JI Jing, WANG Gang. SeNHX1 Protein Localization and Its Function of Na~＋-detoxification[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 8-11. doi: 10.3969/j.issn.1000-7091.2012.05.002.

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