Cloning and Genetic Transformation of Wild Soybean GsGSK1 Gene into Arabidopsis

doi:10.3969/j.issn.1000-7091.2012.05.005

Abstract

Abstract: Using homologous cloning and RT-PCR technology,a glycogen synthase kinase family gene,GsGSK1 was isolated from wild soybean.It is 1 233 bp in length with one ORF of 410 amino acids.Compared the nucleotide sequence with GmGSK,these two genes have11 SNPs,in which 10 SNPs are synonymous mutations,and 1 SNP is non-synonymous mutation,with a homology of 99.1%.Then constructed the recombinant plant expression vector pCAMBIA3300-GsGSK1 and transformed it into arabidopsis by agrobacterium mediated method.T1 transgenic plant had been acquired,which will be supplied the basic materials for further identification of gene function in GsGSK1.

Key words: Glycogen synthase kinase, Wild soybean, Transgenic arabidopsis, Stress tolerance

CLC Number:

S565.1

ZHANG Chun-bao, TAN Hua, ZHAO Li-mei, PENG Hao, Lü Long-shi. Cloning and Genetic Transformation of Wild Soybean GsGSK1 Gene into Arabidopsis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 22-27. doi: 10.3969/j.issn.1000-7091.2012.05.005.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2012.05.005

http://www.hbnxb.net/EN/Y2012/V27/I5/22

References

[1] Embi N, Rylatt D B, Cohen P. Glycogen synthase kinase-3 from rabbit skeletal muscle, separation from cyclic-AMP dependent protein kinase and phosphorylase kinase [J]. Eur J Biochem,1980,107 :519 -527.

[2] Pay A,Jonak C,Bogre L,et al. The MsK family of alfalfa protein kinase genes encodes homologues of shaggy/glycogen synthase kinase-3 and shows differential expression patterns in plant organs and development [J]. Plant Journal,1993,3(6) :847 856.

[3] Bianchi M W, Guivarc'h D, Thomas M,et al. Arabidopsis homologs of the shaggy and GSK-3 protein kinases : molecular cloning and functional expression in Escherichia coli [J]. Mol Gen Genet, 1994,242:337 345.

[4] Dornelas M C, Lejeune B, Dron M, et al. The Arabidopsis SHAGGY-related protein kinase (ASK) gene family: structure, organization and evolution [J]. Gene, 1998, 212:249 257.

[5] Dornelas M C, Wittich P, Von Recklinghausen I, et al. Characterization of three novel members of the Arabidopsis SHAGGY-related protein kinase (ASK) muhigene family [J]. Plant Mol Biol, 1999,39 : 137 147.

[6] Jonak C, Hirt H. Glycogen synthase kinase 3/SHAGGYlike kinases in plants: an emerging family with novel functions [J 1 ~ Trends Plant Science ,2002,7:457 461.

[7] Tichtinsky G, Tavares R, Takvorian A, et al. An evolutionary conserved group of plant GSK-3/shaggy-like protein kinase genes preferentially expressed in developing pollen [J]. Biochim Biophys Acta, 1998,1442:261 273.

[8] Perez-Perez J M,Ponce M R,Micol J L. The UCU1 Arabidopsis gene encodes a SHAGGY/GSK3-like kinase required for cell expansion along the proximodistal axis[J]. Dev Biol,2002,242 : 161 173.

[9] Kim L, Kimmel A R. GSK3, a master switch regulating cell-fate specification, and tumorigenesis [J]. Curr Opin Genet Dev ,2000,10:508 514.

[10] Piao H L, Kyeong T P, Jeong H L, et al. An Arabidopsis GSK3/shaggy-like gene that complements yeast salt stress-sensitive mutants is induced by NaC1 and abscisic acid[J]. Plant Physiology, 1999,119 : 1527 1534.

[11] Piao H L, Lim J H, Kim S J,et al. Constitutive over-expression of AtGSK1 induces NaCl stress responses in the absenee of NaC1 stress and results in enhanced NaCI tolerance in Arabidopsis[J], Plant J,2001,27 :305 314.

[12] Chen G P,Ma W S,Huang Z J,et al. Isolation and characterization of TaGSK1 involved in wheat salt tolerance [J]. Plant Sei ,2003,165 ( 6 ) : 1369 1375.

[13] 徐涛.小麦耐盐突变体TaGSK1基因的分离和鉴定[D].石家庄:河北师范大学,2003.

[14] Koh S, Lee S C, Kim M K, et al. T-DNA tagged knockout mutation of rice OsGSKI, an orthologue of Arabidopsis BIN2,with enhanced tolerance to various abiotic stresses [J]. Plant Mol Biol,2007 ,65 :453 466.

[15] Zhang C ,Zhao H, Liu Y ,et al. Isolation and characterization of a novel glycogen synthase kinase gene, GmGSK, in Glycine max L. that enhances abiotic stress tolerance in Saccbaromyces cerevisiae [J]. Biotechnology Letter, 2010,32(6) :861 866.

[16] 《玉米非生物逆境响应基因ZmASK1的克隆及表达特性分析》[J].作物学报,2008(2).

Please choose a citation manager

Content to export