Expression and Functional Analysis of Maize ZAG3

doi:10.3969/j.issn.1000-7091.2012.05.004

Abstract

Abstract: In this paper,bioinformatical and expression analysis were used to examine the functions of ZAG3,a AGL6-like gene in maize.Sequence analysis indicated that ZAG3 encoded a MADS-box protein with 255 amino acids.ZAG3 protein was predicted to be subcellular located in the nucleus.Furthermore,the promoter sequenced was analysised to contain some important regulatory element,including core promoter elements,meristem expression element,abscisic acid responsiveness and gibberellin-responsiveness element,etc.Noticeable,MeJA-responsive regulatory element was not found in the promoter region,whereas the expression of ZAG3 was remarkable up-regulated in the MeJA-treated tassel by using the Q-PCR technology.These results suggest that ZAG3 gene may relate to the JA-mediated sex determination process in maize tassel.

Key words: Maize, Sex determination AGL6 ZAG3 JA

CLC Number:

S513

LI Hao-ge, LIU Yan-fei, ZHONG Ming, CHEN Li-jing, ZHANG Li, LIN Jing-wei, CUI Zhen-hai. Expression and Functional Analysis of Maize ZAG3[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 18-21. doi: 10.3969/j.issn.1000-7091.2012.05.004.

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