Analysis of NSP2 and GP5 Gene Variation of Porcine Reproductive and Respiratory Virus Strain Isolated in Henan Province

doi:10.3969/j.issn.1000-7091.2012.02.020

Abstract

Abstract: Amplifying the NSP2 and GP5 genes sequences of nine strains PRRSV isolated from different areas?of Henan between 2006 and 2009，these genes of NSP2 exhibited different sizes in length ranging from 2 850 bp to?2 937 bp and coded 950 －979 aa，and the length of GP5 were 603 bp and coded 200 aa． Nucleotide and deduced amino acid sequences analysis revealed that 7 out of 9 Nsp2 sequences contained the same 30 aa deletion and the results indicated that 9 strains were American． In order to compare the consistency between Nsp2 and GP5 variations, the Nsp2 and GP5 genes sequence of nine strains PRRSV genome isolated from Henan were analyzed，then exploring?the genetic variation characteristics of epidemic PRRSV strains．

Key words: Porcine reproductive and respiratory syndrome virus, NSP2 gene, ORF5 gene, Variation sequence

CLC Number:

S852.65

LU Xiao-yan, ZHOU Yong-hui, LI Wei-juan, ZHANG Zhi-yuan, LIU Xiao-ping, DUAN Er-zhen, XIA Ping-an, CUI Bao-an. Analysis of NSP2 and GP5 Gene Variation of Porcine Reproductive and Respiratory Virus Strain Isolated in Henan Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 100-104. doi: 10.3969/j.issn.1000-7091.2012.02.020.

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