Abstract: Specific primers of PRRSV ORF2 to ORF7 were designed and synthesized according to the published gene sequence of PRRSV from GenBank by Primer software, and ORF2, ORF3,ORF4, ORF5,ORF6, ORF7 genes of PRRSV HN-HW strain were obtained by RT-PCR method.The RT-PCR products were cloned into the pGEM-Teasy vector and these recombinant plasmid were transformed into E.coli JM109 competent cell.Identification by enzyme digesting and PCR, the positive clones were sent to sequencing.After sequencing, the sequences of ORF2,ORF3,ORF4,ORF5,ORF6, ORF7 genes of the PRRSV HN-HW isolate were compared with sequences of other different PRRSV strains (ATCC VR- 2332, JX-A1,CH-1a,BJ-4,HB-1,HB-2,HEB-1,HUB-1,HUB-2, SP, P129,MN184A, RespPRRS MLV, Prime Pac, LV) in GenBank by DNAStar software.The results showed that the ORF2-ORF7 sequences of the HN-HW strain shared 88.9 %-94.9 % of nucleotide homology and 82.4 %-96.0 % of amino acid homology with North American genotype ATCC VRR-2332 strain.And they shared only 64.0 %-69.0 % of nucleotide homology and 34.5 %-78.9 % of amino acid homology with Europe genotype LV strain.The phylogenetic trees revealed the PRRSV HN-HW strain was clustered within North American genotype and had close relationship with JXA1,HUB-1,HUB-2 and HEB-1.

Key words: Porcine reproductive and respiratory syndrome virus, HN-HW strain, ORF2 to ORF7, Cloning and analysis

CLC Number: 

• Q78