Variation Analysis of Porcine Reproductive and Respiratory Syndrome Virus ORF5-7 Gene of HB-4(hs)Strain

doi:10.7668/hbnxb.2008.04.008

Abstract

Abstract: In order to offer the theoretical data for prevent and control PRRS that unparalleled outbreak in Hebei. According to the reported complete nucleotide sequence of PRRSV CH-1a and JXA1 inGeneBank,3 pairsof primers were designed to amplify ORF5-7 gene of HB -4( hs)strain with Primer 5. 0 software. The ORF5-7 gene nucleotide se- quenceswere obtained by RT-PCR and sequenced after being cloned directly into the pGM-T vector. The obtained sequences were analyzed and compared with the published complete nucleotide sequences of other strains in GeneBank by DNAstar software. The results showed that the PRRSV HB-4( hs)strainwas clustered within North American genotopy. The ORF5-7 gene of HB- 4( hs)strain showed highest homology of 98. 8%- 99. 5%to the strains were isolated in recent years,showed 91. 9%- 92. 6%identity with the strainswere isolated in last years. The phylogenetic trees revealed the strains were isolat- ed in recent years had close relationshipwith HB -1( sh)strain.

Key words: Porcine reproductive and respiratory syndrome virus, ORF5-7 gene, Heredity and variation analysis

CLC Number:

S432.4+1

LIU Tao, CHEN Sai-juan, SUN Ji-guo, SU Xia, WANG Jiao, ZHAO Ze-kun, ZHANG Ping, FU Qi. Variation Analysis of Porcine Reproductive and Respiratory Syndrome Virus ORF5-7 Gene of HB-4(hs)Strain[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 32-36. doi: 10.7668/hbnxb.2008.04.008.

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