Gene Cloning of Porcine Fc Receptor Gamma Subunit and Its Prokaryotic Expression

doi:10.3969/j.issn.1000-7091.2012.01.007

Abstract

Abstract: mRNA encoding porcine FcR gamma subunit obtained from PAMs of 90-day piglets was transcribed into cDNA through RT-PCR. Recombinant plasmid pET-FcRγ was constructed successfully by inserting the cDNA sequence into pET-32a, and expressed as soluble protein with a molecular weight of about 29 kDa. Following purification, recombinant protein FcRγ-His was used to immune mouse, and polyclonal antibody against it was obtained. Western-blotting indicated that such polyclonal antibody could react specifically with FcRγ-His. Indirect ELISA shows that the titer of the polyclonal antibody is 1∶ 8000, revealing the good immunogenicity of FcRγ-His. All these results provide the foundation for further studying the structure and function of FcR gamma subunit.

Key words: Porcine FcR gamma subunit, Gene clone, Prokaryotic expression, Polyclonal antibody

CLC Number:

S828

ZHANG Zhi-yuan, ZHANG Ji-xi, XU Hong-yun, LIU Xiao-ping, LU Xiao-yan, DUAN Er-zhen, XIA Ping-an, CUI Bao-an. Gene Cloning of Porcine Fc Receptor Gamma Subunit and Its Prokaryotic Expression[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 35-38. doi: 10.3969/j.issn.1000-7091.2012.01.007.

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