Abstract: Phenylalanne ammonial-lyase(PAL) is one of the key enzymes involved in rosmarinic acid biosynthetic pathway. In this study, a PAL gene, named PvPAL, was cloned from Prunella vulgaris at the first time by 3'-RACE-PCR and using the specific primer, which was designed according to the homologus sequences of PAL genes from other plants. The GenBank accession number of PvPAL is JN65446. The length of PvPAL cDNA fragment is 1 306 bp, including a 1 047 bp-length coding sequence, which encoded a 349-amino-acid protein. Sequence multiple-alignment revealed that PvPAL protein had extensive homology with those of other plants as Salvia miltiorrhiza, Rehmannia glutinosa, Scutellaria baicalensis and Agastache rugosa. Phylogenetic tree analysis showed that PvPAL had closest relationship with PALs from Lamiaceae plants than from other plants. Tissue expression analysis indicated that PvPAL expressed in all tissues examined, but highest in roots. The isolation of PvPAL provided basis for further studying the molecular mechanism of rosmarinic acid biosynthesis in P. vulgaris.

Key words: Prunella vulgaris, Phenylalanne ammonial-lyase, Gene cloning: Sequence analysis, Expression

CLC Number: 

• Q78