Abstract: "Clp protease controls ordinary metabolic processes by removing abnormal and toxic proteins or peptides caused by stress.By constructing the cDNA library of different stages seeds of peanut cultivar Luhua 14,we selected a sequence of 1 212 bp with polyA in 3'end.By blastx we found that it was a ClpP gene and had 99% similarity with EZ736390.1 in the Genbank.Based on tender peanut seed cDNA,we designed primers and cloned AhClpP gene.Sequence analysis demonstrates that the cDNA of AhClpP gene has a single open reading frame of 843 bp encoding 280 amino acids.The predicted protein has an molecular mass of 30.9 kDa and an isoelectric point of 9.07.The estimated protein contains a S14_ClpP_2 conserved domain,has no signal peptide and sublocates in mitochontria.Compared with other ClpP members,AhClpP has high homology with its counterpart of Arabidopsis thaliana and Ricinus communis.The cloning of AhClpP gene provides foundation for further study of its biological function and application. "

Key words: Arachis hypogaea L, Caseinolytic protease, Cloning Sequence analyzing

CLC Number: 

• S565.2