Identification and Analysis of the hhdB Gene of Haemophilus parasuis

doi:10.7668/hbnxb.2010.S2.005

Abstract

Abstract: The research aimed to know the virulence-associated factors in pathogenic strains of Haemophilus parasuis.Nine strains(H1-H9) of bacteria were isolated from unsound swine within the different regions epidemic farms in Guangdong province,and the hhdB genes of the isolated strains were identified and analyzed.The results showed that the isolated strains belonged to Haemophilus parasuis by morphological observation,biochemical characterization and PCR identify,then had satellite phenomena and nonhemolytic characteristics.The nucleotide sequence similarities of hhdB genes between the isolated strains,Haemophilus parasuis SH0165 are 97.5%-99.8% and 99% respectively.The amino acid sequence of isolated strains hhdB gene and the bacterial hemolysin activation protein(Actinobacillus minor NM305 and Haemophilus ducreyi 35000HP)were similar.RT-PCR indicated that the hhdB gene was expressed in all of the isolated strains.This study would be helpful for identification of virulence gene and pathogenic mechanism study of Haemophilus parasuis

Key words: Haemophilus parasuis, hhdB gene Cloning Analysis

CLC Number:

S858.28

SONG Shuai, LI Chun-ling, YANG Dong-xia, LI Miao. Identification and Analysis of the hhdB Gene of Haemophilus parasuis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S2): 17-21. doi: 10.7668/hbnxb.2010.S2.005.

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http://www.hbnxb.net/EN/Y2010/V25/IS2/17

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