Genetic Analysis of Genes for Yellow Pigment Content in Shaanxi Wheat Cultivars and Lines

doi:10.7668/hbnxb.2010.04.018

Abstract

Abstract: Color is an important trait exerting significant influences on the appearance of wheat－based products. Phytoene synthase (Psy) gene is a critical gene influencing biosynthetic pathway of yellow pigment (YP) in bread wheat. The aims of this study were to detect the allelic variations of Psy-A1 and Psy-B1 genes in 98 wheat cultivars and lines grown mainly in Shaanxi province using the functional markers YP7A and YP7B developed from the sequence polymorphisms of Psy-A1 gene on chromosome 7A and Psy-B1 gene on chromosome 7B，and to identify the distribution and to discuss the relationship between allelic variations of Psy-A1 and Psy-B1 genes to evaluate the feasibility of the markers for selecting wheat cultivars and lines with either low or high YP content. YP7A. The co-dominant marker of Psy-A1 gene can amplify a 194-bp and a 231-bp fragment in the genotypes with Psy-A1a frequently for high YP content and Psy-A1b mostly for low YP content，r espectively; and YP7B-1，another co-dominant marker of Psy-B1 gene，can amplify a 151-bp PCR fragment in accessions with the medium YP content allele Psy-B1a，and a 156-bp fragment in lower YP content accessions with Psy-B1b; two dominant markers YP7B-2 (428 bp) and YP7B-3 (884 bp) were designed for accessions with Psy-B1c and Psy-B1d，respectively. Allele Psy-B1c was associated with high YP content，but the phenotypic effect of Psy-B1d was not determined due to the limited number of accessions. The detecting results showed that the frequency of the genotype Psy-A1a，Psy-A1b，Psy-B1a，Psy-B1b，Psy-B1c and Psy-B1d is 39. 8%， 60. 2%，24. 5%， 67. 4%，7 . 1% and 1. 0%，respectively. In summary ，cultivars and lines with genotypes Psy- A1b and Psy-B1b should be mainly selected for the improvement and introduction of flour color in Shaanxi wheat cultivars (lines) . The functional markers YP7A and YP7B of phytoene synthase genes ( Psy-A1 and Psy-B1) can be directly used for promoting the breeding efficiency.

Key words: Bread wheat (Triticum aestivum L.), Yellow pigment(YP), Phytoene synthase (Psy) gene, Functional marker, Marker-assisted selection (MAS)

CLC Number:

S12.03

YANG Song-jie, ZHANG Ying-quan, WANG Ru, ZHANG Xiao-ke. Genetic Analysis of Genes for Yellow Pigment Content in Shaanxi Wheat Cultivars and Lines[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 83-87. doi: 10.7668/hbnxb.2010.04.018.

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