Cloning and Sequencing of rDNA ITS Regions in Fusarium oxysporum f.sp.benincasae

doi:10.7668/hbnxb.2010.01.015

Abstract

Abstract: The internal transcribed spacer regions (ITS) of the rDNA from Fusarium oxysporum f.sp.Benincasae were amplified with the polymerase chain reaction through the universal primers and sequenced,meanwhile the ITS of different formae specialis of Fusarium oxysporum for the host to Cucurbitaceae were clustered.The total length of ITS in Fusarium oxysporum f.sp.benincasae was 1 063 bp,including partial 18S rDNA,5.8S rDNA,ITS1,ITS2 and partial 28S rDNA.Cluster results showed that 15 isolates were classified into two groups.Group I included 2 Fusarium oxysporum f.sp.niveum and 2 Fusarium oxysporum f.sp.melonis.Group I included 11 isolates,among them Fusarium oxysporum f.sp. benincasae belonged to the group,the others were 5 Fusarium oxysporum f.sp.melonis,3 Fusarium oxysporum f.sp.niveum,1 Fusarium oxysporum f.p.cucumerinum,1 Fusarium oxysporum f.sp.luffae and 1 Fusarium oxysporum f.sp.Lagenariae,respectively.

Key words: Fatmelon, Fusarium oxysporum f.sp.benincasae, Internal transcribed spacer regions

CLC Number:

S436.5

WANG Cui-xia,XU Jin-ru,XI Yu-lin,BAI Xue-fei,WEN Xiao-lie,DUAN Hui-jun. Cloning and Sequencing of rDNA ITS Regions in Fusarium oxysporum f.sp.benincasae[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 80-83. doi: 10.7668/hbnxb.2010.01.015.

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