Cloning,Prokaryotic Expression and Antiviral Activity of Chicken Interferon-a Gene

doi:10.7668/hbnxb.2009.01.010

Abstract

Abstract: The full length of chicken interferon alpha(ChIFN-a) gene was amplified by polymerase chain reaction(PCR)from the genome DNA of Luoman chicken liver,and then sequenced. The amplified gene fragment was 582 by,ofwhich the 489coding region was subcloned into prokaryotic expression vector pQF30. The recombinant plasmidpQEChIFN a was identified by PCR,enzyme digestion and DNA sequencing. SDS PAGE and Western blot showed that a19. 80 kDa fusion protein was expressed in the form of inclusion body with good immunity. After being denatured and re-natured,the activity of inclusion body was detected by inhibiting the cytopathic effect. The activity of recombinant ChIFN“against vesicular stomatitis virus at Vero cell is 1.16 X10³ U/ mg.

Key words: Chicken, IFN-a, Clone, Prokaryotic expression, Antiviral activity

CLC Number:

S852.5
Q78

CHEN Hong-ying, SONG Ling-yun, CUI Bao-an, HU Gong-zheng, JIA Yan-yan, GUO Xian-po. Cloning,Prokaryotic Expression and Antiviral Activity of Chicken Interferon-a Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 40-43. doi: 10.7668/hbnxb.2009.01.010.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2009.01.010

http://www.hbnxb.net/EN/Y2009/V24/I1/40

References

1 陆承平.兽医微生物「Ml.北京:中国农业出版社，2001: 452.
2 Sekellick M J, Ferrandino A F, Flopkins D A，et al. Chicken inteeron gene:cloning,expression,and analysis [J].Journal of lnteiteron and(Zokine Research,1994,14 (2)：71一79.
3 汪明，吴志光，夏春.肉鸡IFN alp ha基因的克隆、序列分析以及在大肠杆菌中的表达[J].农业生物技术学报，2000,8 (4)：337一381.
4 夏春，汪明，朱凌云，等.惠阳胡须鸡r-a基因克隆和序列分析[J].畜牧兽医学报，2000,31 (6)：563 - 565.
5 夏春，汪明，杨琪，等.丝羽乌骨鸡正、基因克隆和序列分析[J].中国免疫学杂志，2000,16 (9)：508 - 513.
6 Takehara K,Itobayashi K,Ruttanapumma R，et al. Adjuvant ei3ect of chicken inte}eron-gamma for inactivated Salmonella Fnteritidis antigen[J].Journal of Veterinary Medical Science， 2003,65(12)：1337一1341.
7 C,Liu J,Wu Z G, et al. The intetferon-alpha genes from three chicken lines and its etJects on H9N2 influenza viruses [J].Animal Biotechnology,2004,15 (1)：77一88.
8 Rauw F, Lambrecht B，van den Berg T. Pivotal role of ChIFNgamma in the patla genesis and immunosuppression of infectious bursal disease [J].Avian Pathology,2007,36 (5): 367一374.
9 Rubinstein S, Familletti P C, Pestka S. Convenient assay for intetferons [J].Journal of Virology,1981,37(2)：755一758.
10 Haghighi H R, Abdul-Careem M F, Dara R A，et cal. tokine gene expression in chicken cecal tonsils following treatment with probiotics and Salmonella infection[J].Vet- erinary 1VTicrobiology,2008,126(1一3)：225一233.
11 Pfeffer L M,Dinarello C A,Herberman R B，et al. Biological properties of recombinant alpha-inte ferons：40th anniversary of the discovery of inte ferons [J].Cancer Research,1998， 58(12)：2489一2499.
12 Pestka S，Trotta P P. Recombinant alpha-inteferons from naturally occurring genes[J].Introduction Seminars in On- cology,1997,24 (3 Suppl 9)：1一3.

[1]	MA Jinge, ZENG Peijun, WAN Shumin, XIONG Xinwei, WANG Zhangfeng, LIU Jing, XU Jiguo, RAO Yousheng. Screening of Genes and Key Pathways Related to Egg-Laying in Kangle Yellow Chickens Based on Liver Transcriptome Sequencing [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 207-214.
[2]	FENG Qingxiang, ZHU Haxiu, DU Yang, XU Yanru, WANG Chenchen, LIU Xue, LI Dayong, ZHOU Jun, ZHANG Bin. Cloning and Expression Analysis of ANS Gene in Cichorium intybus [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 51-59.
[3]	YIN Dongdong, ZHU Xingxing, LAN Mengdie, PENG Mengling, YIN Lei, DAI Yin, SHEN Xuehuai, WANG Jieru, ZHAO Ruihong, PAN Xiaocheng. *Isolation and Identification of Novel Goose astrovirus* AH-2021 Strain and Polyclonal Antibody Preparation of VP27-VP34 Protein** [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(2): 210-217.
[4]	LIU Yongning, SHAN Yige, PAN Chenfan, LIU Qianlin, LI Yilin, DUAN Sizhang, AN Jian, ZHANG Jianjun. *Prokaryotic Expression of Eimeria tenella AMA1* and Evaluation of Its Immune Protection Effect** [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1): 232-238.
[5]	MA Jinge, CHEN Yujie, LEI Wenjing, XU Qiao, XU Jiguo, XU Jing, RAO Yousheng. Identification of Genes Associated with Body Weight at 8 Weeks Age in Kangle Yellow Chickens Based on Omics Data [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1): 224-231.
[6]	HE Lingyu, QIAO Xian, WANG Xinyue, LI Xianglong. Transcriptome Data Reveals the Specific Deposition of Inosine in Muscle Tissue of Bashang Long-tailed Chickens Regulated by PLA2G4A [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(S1): 329-336.
[7]	ZHANG Yuqian, LÜ Zhihang, LIU Chunhong, LIAN Chunyang, ZHANG Xuelian. *Bioinformatics Analysis,Prokaryotic Expression and Polyclonal Antibody Preparation of ORF2 Protein of Avian hepatitis E virus(CaHEV-GDSZ01)Strain* [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(6): 231-238.
[8]	XUE Qian, LI Guohui, ZHOU Chenghao, JIANG Yixiu, XING Weijie, HAN Wei. *Variations,Bioinformatics and Tissue Expression of CDKN2A* Gene in Wenshang Barred Chicken** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(5): 219-230.
[9]	SHAO Wenxian, WANG Dongmei. Antibody Preparation of TaMAPK5 Protein and Its Expression Characteristics in the Interaction Between Wheat and Puccinia triticina [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(5): 186-192.
[10]	ZHANG Yuanchen, SU Shengying, ZHANG Jiaqi, XUE Shuang, WANG Jingshun. *Cloning and Expression Analysis of MseLYS* from Oriental Armyworm,Mythimna separata(Lepidotera:Noctuidae)** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 206-214.
[11]	GUO Zhaoyang, YIN Yuhang, LIU Yu, XIE Yitong, PEI Yuhe, SONG Xiyun, ZHAO Meiai. *Cloning and Expression Pattern Analysis of ZmPAP26b* Gene in Maize under Drought Stress** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 10-19.
[12]	ZHANG Yuqian, LI Changle, LÜ Zhihang, YAO Xinyan, ZHANG Xuelian. *Prokaryotic Expression and Polyclonal Antibody Preparation of ORF3 Protein of Avian hepatitis E virus* CaHEV-GDSZ01 Strain** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 224-229.
[13]	LI Yanxiao, WANG Lina, ZHU Guishuang, LIU Peng, XIANG Dianjun. *Cloning and Expression Characteristics Analysis of RcNAC100-like* Gene in Castor** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 67-76.
[14]	FU Xi, ZHAO Wei, YU Baojun, ZHANG Tong, CHEN Yafei, MAO Xiangyuan, WEI Chuanhao, MA Xiaoping, ZHANG Xinyu, ZHANG Juan. *The Effect of Down-regulation of BPGM* on the Growth and Development of Myogenic Cells in Jingyuan Chickens** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(2): 219-230.
[15]	WANG Huizhen, ZHANG Chaozheng, HUANG Yiming, LI Yaoxin, CHENG Ziyang, YUE Chaoyin. *Cloning and Expression Analysis of Disease Resistance Gene SbRPM1* in Sorghum** [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(2): 153-160.

Please choose a citation manager

Content to export