Cloning of E Gene of PRRS Virus and Construction of Its Expressing Vectors

doi:10.3321/j.issn:1000-7091.2006.01.007

Abstract

Abstract: The E gene fragments of PRRS strain BJ-4 have been amplified and cloned into plasmid pBV221 and pPIC9K.Recombinant plasmid was constructed and transformed into the JM109.The result indicated that the recombinant vectors were successfully constructed.Positive clones of target genes were screened by PCR and identified with restriction endo-nuclease analysis.The positive clone of recombinant prokaryotic expression vector was induced and expressed with ELISA showed that it could react with the antibody to PRRSV.The results showed that E Protein of PRRSV was expressed in prokaryote system.The prokaryotic expression vector and eucaryotic expression vector were successfully constructed,which would be useful for production of gene vaccination in future.

Key words: PRRSV, Gene cloning, Prokaryotic expression, Eucaryotic expression

CLC Number:

Q342

YUAN Bo-hua, JIANG Ji-zhi, LIU Hong-mei. Cloning of E Gene of PRRS Virus and Construction of Its Expressing Vectors[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(1): 27-30. doi: 10.3321/j.issn:1000-7091.2006.01.007.

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