Optimization of Medium for Bacil lus thuringiensis HBF- 1 with Insecticidal Activity to Scarabaeoidae Larva

doi:10.3321/j.issn:1000-7091.2003.z1.027

Abstract

Abstract: Through orthogone experiment,the opt imal medium was obtained for Bacil lus thur inginesisHBF-1 with insect icidal act ivity to scarabaeoidae larva. The medium contained 4value4% nitrogen B,1value2% starch,0value5% yeast power,0value04% KH2PO4,0value05% MgSO4valueFermentation was done with this medium in shakingflask. Result s show ed that the number of cells was 44 value 108cfu/ mL,and the produce of crystal was 2value1130 mg/mL. The mortality of A nomala corp ulenta larv a t reated with 50-fold of HBF- 1 fermented liquid was 78value3%on the 14th day of t reatment.

Key words: Bacillus thur ingiensis, HBF-1 st rain, Scarabaeoidae larva, Fermentation, Orthogonal experiment

CLC Number:

S476+.11 ??

WANG Rong-yan, FENG Shu-liang, FAN Xiu-hua, CAO Wei-ping, HU Ming-jun. Optimization of Medium for Bacil lus thuringiensis HBF- 1 with Insecticidal Activity to Scarabaeoidae Larva[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3321/j.issn:1000-7091.2003.z1.027.

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