Double Color FISH of rRNA Genes in Wheat Relatives of H.villosa and Th.ihtermedium

doi:10.3321/j.issn:1000-7091.2002.01.002

Abstract

Abstract: Haynaldia villosa(2n=14,VV)and Thinopyrum intermedium(2n=42,E₁E₁E₂E₂StSt)are important wheat relatives providing useful resistant genes for wheat improvement.In this paper, rRNA genes coding 25S-5. 8S-18S rRNA and SS rRNA are visualized on H.villosa and Th.intermedium chromosomes by double color fluorescence in situ hybridization. The 25S-5.8S-18S rRNA genes are localized on the short arm of 1V chromosome,and 5S rRNA genes are on the short arm of SV chromosome of H.villasa.On the other hand, three pairs of the 25S-5.8S-18S rRNA genes and four pairs of the SS rRNA genes are observed on the Th.intermedium chromosomes,in which 2 pairs of chromosomes have both rRNA genes on their short arms.

Key words: Haynaldia villosa, Thinopyrum intermedium, rRNA genes, Two color FISH

CLC Number:

Q943

Pei Ziyou, Yuan Wenye, Sun Shancheng, Sun Yu, M Tomita, Y Yasumuro. Double Color FISH of rRNA Genes in Wheat Relatives of H.villosa and Th.ihtermedium[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(1): 6-10. doi: 10.3321/j.issn:1000-7091.2002.01.002.

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