Studies on Enhancement of Transgenic Potato＇s Resistance to Late-blight by Inducing the Expression of Thaumatin-like Protein Gene

doi:10.3321/j.issn:1000-7091.2001.01.013

Abstract

Abstract: Thaumat in??like protein ( TLP) gene and bar gene which is closely combined with TLP gene were transferred into virus free micropotato variety Jing Yinshu 8 by agrobacterium??mediated transformat ion. Transgenic plants selected by bialaphos were amplified for detection. There sults of PCR of maker bar gene and Southern blot of specific probe with TLP gene showed that transgenic plants displayed 0.54 kb and 3.0 kb bands, respectively, indicating TLP gene has been successfully integ rated into the chromosome of transgenic potatoes. The western reaction of trans genic plants using TLP antiserum showed the expression of TLP geneint ransg enic plans was much higher than the control. Through the vitro assay of leaves inoculated by alive spores of phytophthora, leaves of transgenic potato plant s exhibited significant inhibition on late blight disease and delayed development of disease symptoms.

Key words: Potato late-blight, Thaumat in-like protein(TLP) gene, Expression, Resistance

CLC Number:

S532.035.3

JIN Hong, YUE Dongxia, ZHOU Liangyan, S.Muthukrishnan. Studies on Enhancement of Transgenic Potato＇s Resistance to Late-blight by Inducing the Expression of Thaumatin-like Protein Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(1): 67-72. doi: 10.3321/j.issn:1000-7091.2001.01.013.

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