A Preliminary Study by Using Seed Soaking Method of Introduce Exogenous DNA to Induce the Genetic Characters in Flue-cured Tobacco(Ⅱ)

doi:10.3321/j.issn:1000-7091.2001.01.014

Abstract

Abstract: In this experiment,the variant materials obtained by seed soaking method were tested for biochemical indices such as chlorophyll,soluble sugar,reducing sugar,protein and Vitamin C contents,as well as plant height,plant type,leaf area and growth period.The results showed that plant height,plant type,leaf area,growth period and disease resistance were significantly affected. However,the contents of chlorophyll,soluble sugar ,reducing sugar,protein and Vitamin C were not.The method has the advantages of high ratio of variation(21.2%),easy to operate,and the separations stablize quickly.

Key words: Flue-cured tobacco, Seed soaking method, Exogenous DNA Introduction, Variance

CLC Number:

S72.01

ZHU Shengwei, XU Zhong, ZHANG Hanshuang, SHI Zhiwen. A Preliminary Study by Using Seed Soaking Method of Introduce Exogenous DNA to Induce the Genetic Characters in Flue-cured Tobacco(Ⅱ)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(1): 73-77. doi: 10.3321/j.issn:1000-7091.2001.01.014.

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