Abstract:

To understand the germplasm characteristics of Schizothorax prenanti and provide a theoretical basis for its germplasm identification,traditional morphological methods were employed to observe the morphological features,and polyacrylamide gel electrophoresis was used to study the characteristics of lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in the heart,muscle,eye lens,liver,and kidney tissues.The results showed that the main morphological characteristics of S.prenanti were as follows:elongated body with a slightly laterally compressed shape;inferior mouth with a transverse fissure and a blunt,rounded snout;a sharp keratinous edge on the anterior margin of the lower jaw,one pair of barbels on the snout and one pair at the corners of the mouth,with the latter located posterior to the former;dorsal fin formula D.ⅲ-8,with the undivided fin rays of the dorsal fin in sample fish being weak and having a smooth posterior margin or only a few serrations;pectoral fin formula P.i-18-20,pelvic fin formula V.i-9-10,and anal fin formula A.ⅲ-5,with a row of large scales on each side of the anal fin and anus;a two-chambered swim bladder,with the posterior chamber longer than the anterior one;a black peritoneum;a total of 4+40-45 vertebrae;three rows of pharyngeal teeth with a dental formula of 2·3·5/5·3·2.The expression of LDH and MDH isozymes in S.prenanti exhibited tissue specificity,with the expression patterns in different tissues closely related to their physiological functions.LDH expression in the liver was encoded by three genes,namely LDHA,LDHB,and LDHC,and the enzyme band encoded by LDHC tending towards the cathode.There were five LDH enzyme bands in the eye lens,with stable expression.Therefore,it is recommended to use LDH in the eye lens as a biochemical genetic marker for germplasm identification of S.prenanti.

Key words: Schizothorax prenanti, Morphological characteristics, Lactate dehydrogenase, Malate dehydrogenase, Germplasm identification

CLC Number: 

• S917.4水产动物学