Comparative Analysis of Drought Resistance of Luyu 13 Parents

doi:10.7668/hbnxb.20193524

Abstract

Abstract:

Luyu 13 is an excellent maize hybrid bred by the Millet Research Institute of Shanxi Agricultural University.It has strong drought and stress tolerance,and has achieved remarkable social benefits.In order to compare the drought and stress tolerance characteristics of the parents 1572 and Hai 921 of maize hybrid Luyu 13,we analyzed the related parameters of seed germination and seedling growth under simulated drought stress with 20% PEG solution.Results showed that there was no significant difference in the germination parameters between Luyu 13,1572 and Hai 921 under normal conditions,but the germination rate,germination energy,germination index and sprout index were inhibited under drought conditions,while the inhibition degree of Hai 921 was significantly higher than that of 1572 and Luyu 13.These results showed that the seed vigor of 1572 was higher than that of Hai 921,indicating inbred line 1572 had higher germination rate and germination advantage under drought stress,and possess stronger adaptability to drought.Subsequently,the important stresses response genes,ZmCIPK16 and ZmSAM1,were selected and analyzed their expression in 1572 and Hai 921 inbred lines under different abiotic stresses at seedling stage and under drought stress at different growth stages by Real-time PCR.Results showed that ZmCIPK16 and ZmSAM1 were widely responses to drought,salt,low temperature,high temperature,and ABA at seedling stage of 1572 and Hai 921 and responses to drought at different growth stages.ZmCIPK16 and ZmSAM1 showed more actively participated in the response to drought and other stresses in 1572.These results indicated that the inbred line 1572 was an excellent germplasm with drought and stress tolerance,and its contribution to drought resistance of hybrid Luyu 13 was greater than that of inbred line Hai 921.

Key words: Luyu 13, Inbred line 1572, Drought, Gene expression

ZHANG Wenzhong, ZHAO Jinfeng, LU Ming, WANG Huihui. Comparative Analysis of Drought Resistance of Luyu 13 Parents[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(1): 117-123. doi: 10.7668/hbnxb.20193524.

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References 28

[1]	崔静宇, 关小康, 杨明达, 刘影, 丁超明, 李玉华, 王同朝. 基于主成分分析的玉米萌发期抗旱性综合评定[J]. 玉米科学, 2019, 27(5):62-72.doi:10.13597/j.cnki.maize.science.20190510. doi: 10.13597/j.cnki.maize.science.20190510
	Cui J Y, Guan X K, Yang M D, Liu Y, Ding C M, Li Y H, Wang T C. Comprehensive evaluation of maize drought resitance in maize germination period based on principal component analysis[J]. Journal of Maize Sciences, 2019, 27(5):62-72.
[2]	Wang W X, Vinocur B, Altman A. Plant responses to drought,salinity and extreme temperatures:Towards genetic engineering for stress tolerance[J]. Planta, 2003, 218(1):1-14.doi:10.1007/s00425-003-1105-5. doi: 10.1007/s00425-003-1105-5 URL
[3]	Faghani E, Gharechahi J, Komatsu S, Mirzaei M, Khavarinejad R A, Najafi F, Farsad L K, Salekdeh G H. Data in support of comparative physiology and proteomic analysis of two wheat genotypes contrasting in drought tolerance[J]. Data Brief, 2015, 2: 26-28. doi:10.1016/j.dib.2014.11.001. doi: 10.1016/j.dib.2014.11.001 pmid: 26217700
[4]	王秋兰, 靳鲲鹏, 刘永忠, 李万星, 曹晋军, 李丹, 李小霞. 玉米苗期抗旱性鉴定指标及综合评价[J]. 山西农业科学, 2019, 47(3):319-322,365.doi:10.3969/j.issn.1002-2481.2019.03.07. doi: 10.3969/j.issn.1002-2481.2019.03.07
	Wang Q L, Jin K P, Liu Y Z, Li W X, Cao J J, Li D, Li X X. Identification index and comprehensive evaluation of drought resistance in maize seedling stage[J]. Journal of Shanxi Agricultural Sciences, 2019, 47(3):319-322,365.
[5]	张仁和, 马国胜, 卜令铎, 史俊通, 薛吉全. 不同基因型玉米品种抗旱性鉴定及综合评价[J]. 种子, 2009, 28(10):91-94.doi:10.3969/j.issn.1005-0906.2006.01.030. doi: 10.3969/j.issn.1005-0906.2006.01.030
	Zhang R H, Ma G S, Bu L D, Shi J T, Xue J Q. Appraisition and comprehensive evaluation of different genotype maize cultivars for drought resistance[J]. Seed, 2009, 28(10):91-94.
[6]	赵长江, 都梦翔, 宋巨奇, 徐尚缘, 贺琳, 徐晶宇, 杨克军, 李佐同. 玉米NRL基因家族鉴定与逆境表达分析[J]. 华北农学报, 2022, 37(4):1-10.doi:10.7668/hbnxb.20192757. doi: 10.7668/hbnxb.20192757
	Zhao C J, Du M X, Song J Q, Xu S Y, He L, Xu J Y, Yang K J, Li Z T. Genome-wide identification and expression response to stresses analysis of NRL gene family in zea mays[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4):1-10.
[7]	崔蓉, 王天野, 王呈玉, 李锦秀, 张欣宇, 刘淑霞. 干旱胁迫对不同玉米品种生长性状及产量的影响[J]. 华北农学报, 2021, 36(S1):94-100.doi:10.7668./hbnxb.20192242. doi: 10.7668/hbnxb.20192242
	Cui R, Wang T Y, Wang C Y, Li J X, Zhang X Y, Liu S X. Effects of drought stress on growth characters and yield of different maize varieties[J]. Acta Agriculturae Boreali-Sinica, 2021, 36(S1):94-100. doi: 10.7668/hbnxb.20192242
[8]	Xu J, Yuan Y B, Xu Y B, Zhang G Y, Guo X S, Wu F K, Wang Q, Rong T Z, Pan G T, Cao M J, Tang Q L, Gao S B, Liu Y X, Wang J, Lan H, Lu Y L. Identification of candidate genes for drought tolerance by whole-genome resequencing in maize[J]. BMC Plant Biology, 2014, 14:83.doi:10.1186/1471-2229-14-83. doi: 10.1186/1471-2229-14-83 pmid: 24684805
[9]	宋殿珍, 张文忠, 刘景秀, 栗红生, 杨国英, 赵晋峰. 中晚熟玉米单交种潞玉13的选育报告[J]. 玉米科学, 2005, 13(S1):79-81.doi:10.3969/j.issn.1005-0906.2005.z1.037. doi: 10.3969/j.issn.1005-0906.2005.z1.037
	Song D Z, Zhang W Z, Liu J X, Li H S, Yang G Y, Zhao J F. Breeding report of middle and late maturity maize single cross variety Luyu 13[J]. Journal of Maize Sciences, 2005, 13(S1):79-81.
[10]	李中青, 李齐霞, 宋殿珍, 孙万荣, 霍成斌. 潞玉13玉米杂交种丰产性、稳产性及适应性分析[J]. 吉林农业科学, 2008, 33(1):7-9.doi:10.3969/j.issn.1003-8701.2008.01.002. doi: 10.3969/j.issn.1003-8701.2008.01.002
	Li Z Q, Li Q X, Song D Z, Sun W R, Huo C B. Analysis on crop productivity,stability and adaptability of maize hybrid Luyu 13[J]. Journal of Jilin Agricultural Sciences, 2008, 33(1):7-9.
[11]	张文忠, 郭国亮, 宋殿珍, 李洪, 李中青, 芦明, 王慧慧. 玉米自交系1572选育与应用[J]. 安徽农业科学, 2019, 47(19):33-35.doi:10.3969/j.issn.0517-6611.2019.19.011. doi: 10.3969/j.issn.0517-6611.2019.19.011
	Zhang W Z, Guo G L, Song D Z, Li H, Li Z Q, Lu M, Wang H H. Breeding and application of maize inbred line 1572[J]. Journal of Anhui Agricultural Sciences, 2019, 47(19):33-35.
[12]	Zhao J F, Sun Z F, Zheng J, Guo X Y, Dong Z G, Huai J L, Gou M Y, He J G, Jin Y S, Wang J H, Wang G Y. Cloning and characterization of a novel CBL-interacting protein kinase from maize[J]. Plant Molecular Biology, 2009, 69(6):661-674.doi:10.1007/s11103-008-9445-y. doi: 10.1007/s11103-008-9445-y pmid: 19105030
[13]	余爱丽, 赵晋锋, 王高鸿, 杜艳伟, 李颜方, 张正. 玉米ZmSAMS1基因在盐、干旱等逆境胁迫下的表达分析[J]. 玉米科学, 2016, 24(3):31-35.doi:10.13597/j.cnki.maize.science.20160306. doi: 10.13597/j.cnki.maize.science.20160306
	Yu A L, Zhao J F, Wang G H, Du Y W, Li Y F, Zhang Z. Expression analysis of maize ZmSAMS1 gene under salt,drought and other stresses[J]. Journal of Maize Sciences, 2016, 24(3):31-35.
[14]	任杨柳, 乔大河, 胡春辉, 张龙, 李玉玲. 不同种质玉米自交系种子萌发的干旱胁迫[J]. 中国农学通报, 2017, 33(9):17-21.doi:10.3969/j.issn.1001-8581.2016.03.007. doi: 10.11924/j.issn.1000-6850.casb16120083
	Ren Y L, Qiao D H, Hu C H, Zhang L, Li Y L. Drought stress on different maize inbred lines at germination stage[J]. Chinese Agricultural Science Bulletin, 2017, 33(9):17-21. doi: 10.11924/j.issn.1000-6850.casb16120083
[15]	许健, 于海林, 孙善文, 王俊强, 韩业辉, 于运凯, 周超, 兰宏宇, 丁昕颖. PEG模拟干旱胁迫对不同品种玉米萌发期的影响[J]. 中国种业, 2021(1):61-64.doi:10.3969/j.issn.1671-895x.2021.01.019. doi: 10.3969/j.issn.1671-895x.2021.01.019
	Xu J, Yu H L, Sun S W, Wang J Q, Han Y H, Yu Y K, Zhou C, Lan H Y, Ding X Y. Effects of PEG simulated drought stress on germination period of different maize varieties[J]. China Seed Industry, 2021(1):61-64.
[16]	赵晋锋, 杜艳伟, 王高鸿, 李颜方, 赵根有, 王振华, 成凯, 王玉文, 余爱丽. 谷子NADP-ME的鉴定及其对逆境胁迫的响应[J]. 中国农业科学, 2019, 52(22):3950-3963.doi:10.3864/j.issn.0578-1752.2019.22.002. doi: 10.3864/j.issn.0578-1752.2019.22.002
	Zhao J F, Du Y W, Wang G H, Li Y F, Zhao G Y, Wang Z H, Cheng K, Wang Y W, Yu A L. Identification NADP-ME gene of foxtail millet and its response to stress[J]. Scientia Agricultura Sinica, 2019, 52(22):3950-3963. doi: 10.3864/j.issn.0578-1752.2019.22.002
[17]	Sambrook J, Russell D W. Molecular cloning:a laboratory manual[M]. 3rd ed. New York: Cold Spring Harbor Laboratory Press, 2001.
[18]	Livak K J, Schmittgen T D. Analysis of relative gene expression data using Real-time quantitative PCR and the 2^-ΔΔCTmethod[J]. Methods, 2001, 25(4):402-408.doi:10.1006/meth.2001.1262. doi: 10.1006/meth.2001.1262 pmid: 11846609
[19]	余爱丽, 赵晋锋, 王高鸿, 杜艳伟, 李颜方, 张正. 玉米SAMS家族基因鉴定及其在逆境胁迫下的表达分析[J]. 中国农学通报, 2016, 32(8):30-36.doi:10.3724/SP.J.1006.2011.01012. doi: 10.11924/j.issn.1000-6850.casb15090139
	Yu A L, Zhao J F, Wang G H, Du Y W, Li Y F, Zhang Z. Identification of SAMS genes from maize and its expression under adversity stresses[J]. Chinese Agricultural Science Bulletin, 2016, 32(8):30-36. doi: 10.11924/j.issn.1000-6850.casb15090139
[20]	Kevbrin V V, Zengler K, Lysenko A M, Wiegel J. Anoxybacillus kamchatkensis sp.nov.,a novel thermophilic facultative aerobic bacterium with a broad pH optimum from the Geyser valley,Kamchatka[J]. Extremophiles, 2005, 9(5):391-398.doi:10.1007/s00792-005-0479-7. doi: 10.1007/s00792-005-0479-7 pmid: 16142505
[21]	Mato J M, Alvarez L, Ortiz P, Pajares M A. S-adenosylmethionine synthesis:Molecular mechanisms and clinical implications[J]. Pharmacology & Therapeutics, 1997, 73(3):265-280.doi:10.1016/s0163-7258(96)00197-0. doi: 10.1016/s0163-7258(96)00197-0
[22]	柴兴苹, 张玉秀, 谭金娟, 冯珊珊, 柴团耀. Zn胁迫下小麦S-腺苷甲硫氨酸代谢途径关键基因表达模式分析[J]. 植物生理学报, 2013, 49(4):375-384.doi:10.13592/j.cnki.ppj.2013.04.009. doi: 10.13592/j.cnki.ppj.2013.04.009
	Chai X P, Zhang Y X, Tan J J, Feng S S, Chai T Y. Analysis of expression patterns of genes participated in S-adenosylmethionine(SAM)metabolic pathway in wheat under Zn stress[J]. Plant Physiology Journal, 2013, 49(4):375-384.
[23]	樊金萍, 柏锡, 李勇, 纪巍, 王希, 才华, 朱延明. 野生大豆S-腺苷甲硫氨酸合成酶基因的克隆及功能分析[J]. 作物学报, 2008, 34(9):1581-1587.doi:10.3321/j.issn:0496-3490.2008.09.014. doi: 10.3321/j.issn:0496-3490.2008.09.014
	Fan J P, Bai X, Li Y, Ji W, Wang X, Cai H, Zhu Y M. Cloning and function analysis of gene SAMS from Glycine soja[J]. Acta Agronomica Sinica, 2008, 34(9):1581-1587. doi: 10.3724/SP.J.1006.2008.01581 URL
[24]	Luan S. The CBL-CIPK network in plant calcium signaling[J]. Trends in Plant Science, 2009, 14(1):37-42.doi:10.1016/j.tplants.2008.10.005. doi: 10.1016/j.tplants.2008.10.005 pmid: 19054707
[25]	Guo Y, Halfter U, Ishitani M, Zhu J K. Molecular characterization of functional domains in the protein kinase SOS2 that is required for plant salt tolerance[J]. The Plant Cell, 2001, 13(6):1383-1400.doi:10.1105/tpc.13.6.1383. doi: 10.1105/tpc.13.6.1383 URL
[26]	Batistic O, Kudla J. Integration and channeling of calcium signaling through the CBL calcium sensor/CIPK protein kinase network[J]. Planta, 2004, 219(6):915-924.doi:10.1007/s00425-004-1333-3. doi: 10.1007/s00425-004-1333-3 pmid: 15322881
[27]	赵晋锋, 余爱丽, 王高鸿, 田岗, 王寒玉, 杜艳伟, 常海霞. 植物CBL/CIPK网络系统逆境应答研究进展[J]. 中国农业科技导报, 2011, 13(4):32-38.doi:10.3969/j.issn.1008-0864.2011.04.05. doi: 10.3969/j.issn.1008-0864.2011.04.05
	Zhao J F, Yu A L, Wang G H, Tian G, Wang H Y, Du Y W, Chang H X. Progress of CBL/CIPK signal system in response to stresses in plant[J]. Journal of Agricultural Science and Technology, 2011, 13(4):32-38. doi: 10.3969/j.issn.1008-0864.2011.04.05
[28]	严杰, 陶琴君, 陈静, 王强, 汤福蓉, 鲁旺, 张燕. 新选玉米自交系耐盐性鉴定及耐盐机理浅析[J]. 广东农业科学, 2013, 40(8):8-10,21.doi:10.3969/j.issn.1004-874x.2013.08.003. doi: 10.3969/j.issn.1004-874x.2013.08.003
	Yan J, Tao Q J, Chen J, Wang Q, Tang F R, Lu W, Zhang Y. Salt-tolerance indefication of new maize inbred lines and preliminary study on the mechanism of salt-tolerance[J]. Guangdong Agricultural Sciences, 2013, 40(8):8-10,21.

品种名称 Varieties	发芽率/% Germination rate		发芽势/% Germination energy		发芽指数 Germination index		萌发指数 Sprout index
品种名称 Varieties	对照 CK	干旱 Drought	对照 CK	干旱 Drought	对照 CK	干旱 Drought	对照 CK	干旱 Drought
潞玉13 Luyu 13	0.99±0.02a	0.86±0.04b	0.64±0.04a	0.44±0.09b	4.85±0.35a	4.12±0.38b	1.26±0.17a	0.80±0.16b
1572	0.98±0.02a	0.85±0.04b	0.65±0.06a	0.42±0.09b	4.66±0.35a	3.97±0.42c	1.25±0.14a	0.78±0.16b
海921 Hai 921	0.97±0.03a	0.68±0.05c	0.68±0.07a	0.27±0.05c	4.57±0.26a	2.34±0.23d	1.23±0.13a	0.52±0.15c

品种名称 Varieties	发芽率/% Germination rate		发芽势/% Germination energy		发芽指数 Germination index		萌发指数 Sprout index
品种名称 Varieties	对照 CK	干旱 Drought	对照 CK	干旱 Drought	对照 CK	干旱 Drought	对照 CK	干旱 Drought
潞玉13 Luyu 13	0.99±0.02a	0.86±0.04b	0.64±0.04a	0.44±0.09b	4.85±0.35a	4.12±0.38b	1.26±0.17a	0.80±0.16b
1572	0.98±0.02a	0.85±0.04b	0.65±0.06a	0.42±0.09b	4.66±0.35a	3.97±0.42c	1.25±0.14a	0.78±0.16b
海921 Hai 921	0.97±0.03a	0.68±0.05c	0.68±0.07a	0.27±0.05c	4.57±0.26a	2.34±0.23d	1.23±0.13a	0.52±0.15c

品种名称 Varieties	株高/cm Plant height		根长/cm Root length		单株地上干质量/g Aboveground dry weight per plant		单株地下干质量/g Underground dry weight per plant		根冠比 Root shoot ratio
品种名称 Varieties	对照 CK	干旱 Drought	对照 CK	干旱 Drought	对照 CK	干旱 Drought	对照 CK	干旱 Drought	对照 CK	干旱 Drought
潞玉13 Luyu 13	17.36±0.38a	14.27±0.58c	14.35±0.43a	12.34±0.37b	0.079±0.004a	0.054±0.002cd	0.053±0.028a	0.042±0.052c	0.671±0.056bc	0.793±0.043a
1572	15.36±0.58bc	12.35±0.74d	12.38±0.52b	10.23±0.32c	0.072±0.005ab	0.051±0.004d	0.048±0.032b	0.038±0.052d	0.667±0.074c	0.745±0.063ab
海921 Hai 921	16.23±0.54ab	11.26±0.65d	11.32±0.56bc	8.45±0.36d	0.062±0.006bc	0.026±0.003e	0.042±0.036c	0.018±0.013e	0.677±0.042c	0.692±0.132b

品种名称 Varieties	株高/cm Plant height		根长/cm Root length		单株地上干质量/g Aboveground dry weight per plant		单株地下干质量/g Underground dry weight per plant		根冠比 Root shoot ratio
品种名称 Varieties	对照 CK	干旱 Drought	对照 CK	干旱 Drought	对照 CK	干旱 Drought	对照 CK	干旱 Drought	对照 CK	干旱 Drought
潞玉13 Luyu 13	17.36±0.38a	14.27±0.58c	14.35±0.43a	12.34±0.37b	0.079±0.004a	0.054±0.002cd	0.053±0.028a	0.042±0.052c	0.671±0.056bc	0.793±0.043a
1572	15.36±0.58bc	12.35±0.74d	12.38±0.52b	10.23±0.32c	0.072±0.005ab	0.051±0.004d	0.048±0.032b	0.038±0.052d	0.667±0.074c	0.745±0.063ab
海921 Hai 921	16.23±0.54ab	11.26±0.65d	11.32±0.56bc	8.45±0.36d	0.062±0.006bc	0.026±0.003e	0.042±0.036c	0.018±0.013e	0.677±0.042c	0.692±0.132b

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