Study on Breeding and Physiological and Biochemical Characteristics of Cotton with Different Hair Traits of Near Isogenic Lines

doi:10.7668/hbnxb.2017.03.021

Abstract

Abstract: To cultivate new cotton varieties with fine character against diseases and insect pests and locate and clone the trichome gene,near-isogenic cotton lines with smooth and trichome were bred. Photosynthetic rates,chlorophyll amounts,phenylalanine ammonialyase (PAL) activities and tannin levels were studied to disclose the differences between physiological and biochemical chartacteristics on two different trichome near-isogenic cotton lines through the photosynthetic physiology combined with related biochemical index determination. The results showed that chlorophyll a,b and total amount dynamic change trend were consistent between two cotton near-isogenic lines with higher amount at bud stage and lower at flowering and boll stage. Net photosynthetic rate change trend (up-down-up) were also the same. Therefore photosynthetic rates and chlorophyll amounts had the same change trend and their differences were not obvious between the materials.Tannin contents between two lines in fiber and seed coat changed with the same trend-from high to low. Fiber PAL activities decreased in thichome but decreased and then rised in smooth. Testa PAL activities tised and then remained unchanged. PAL activities and tannin levels were evidently different with trichome cotton more than the smooth. It was speculated that PAL activities and tannin levels were associated with the trichome trait.

Key words: Near-isogenic line, Trichome trait, Chlorophyll amounts, Phenylalanine ammonialyase (PAL), Tannin

CLC Number:

S562.01

ZHANG Jinlong, LIU Yi, QU Yunfang, KANG Nana, DU Haiye, HUANG Jinling. Study on Breeding and Physiological and Biochemical Characteristics of Cotton with Different Hair Traits of Near Isogenic Lines[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(3): 137-142. doi: 10.7668/hbnxb.2017.03.021.

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References

[1] 杨红旗,崔卫国.我国棉花产业形势分析与发展策略[J].作物杂志, 2010(5):13-17.
[2] 赵冰梅,李贤超,王俊刚.2011年新疆兵团棉花病虫害发生特点及原因分析[J].中国棉花,2012,39(3):9-11.
[3] 郭宝德,姜艳丽,冀丽霞,等. Bt基因导入形态抗蚜棉研究[J]. 山西农业科学,2013,4l(11):117l-1173.
[4] 何叔军,赵瑞元,杨晓萍. 亚华棉10号在湖南试种表现及高产栽培技术要点[C]//中国棉花学会2009年年会论文汇编. 北京:中国棉花学会,2009:280-281.
[5] 李丹.棉花黄萎病抗性评价指标与抗病相关基因引物分析[D].乌鲁木齐:新疆农业大学,2014.
[6] 宋培玲,张键, 郝丽芬,等.不同抗性油菜品种接种黑胫病菌防御酶活性变化研究[J].华北农学报, 2015, 30(2):110-115.
[7] 张笑宇,于肖夏,于卓,等.黑痣病菌毒素诱导马铃薯幼苗体内防御酶活性的变化[J].华北农学报,2012,27(4):153-157.
[8] 杜欣谊.苯丙氨酸解氨酶的研究进展[J].现代化农业, 2016(7):24-26.
[9] 林凤敏,吴敌,陆宴辉,等.棉花主要抗虫次生物质与其对绿盲蝽抗性的关系[J].植物保护学报, 2011, 38(3):202-208.
[10] 阮志平,向平.植物单宁对微生物的抑制作用及其机制[J].华北农学报,2006,21(10):16-19.
[11] 徐刚红,沈万宽,吴夏明,等.不同遗传类群甘蔗黑穗病菌分离物与甘蔗互作防御酶差异的研究[J].华北农学报, 2016, 31(2):218-223.
[12] 王妍君,谢开云,赵祥,等.植物单宁及其对动物的作用研究进展[J].草原与草坪, 2011,31(4):82-86.
[13] 申宏波,胡志凤,丁俊杰,等.Harpins诱导苯丙氨酸解氨酶(PAL)活性防治大豆疫霉根腐病研究[J].大豆科学, 2011, 30(3):526-528.
[14] 李炳林,张伯静,张新润.亚洲棉与比克氏棉杂交的研究[J].遗传学报,1987(2):165-166.
[15] 李炳林,祝水金,王红梅.种子无腺体植株有腺体棉花异源四倍体新种质的育成和研究[J].棉花学报,1991,3(1):27-32.
[16] 张伯静,李炳林,祝水金.亚洲棉,比克氏棉和陆地棉异源四倍体的合成[J].棉花学报,1993,5(1):15-20.
[17] 李得孝,郭月霞,员海燕,等.玉米叶绿素含量测定方法研究[J].中国农学通报,2005,21(6):153-155.
[18] 武予清;郭予元.棉花植株中的单宁测定方法研究[J].应用生态学报,2000,11(2):243-245.
[19] 欧阳光察; 薛应龙.植物苯丙烷类代谢的生理意义及其调控[J].植物生理学通讯,2008(3):9-26.
[20] 余渝,陈冠文,田笑明,等.新疆棉花叶光合速率的变化特点研究[J].新疆农业大学学报,2001,24(1):16-20.
[21] 吴春太,苏运,魏孝民,等.橡胶树无性系叶绿素含量与光合速率和白粉病抗性的关系[J].湖南农业大学学报:自科版, 2011, 37(4):381-384.
[22] 宋莎,江旭生,韩秀梅,等.不同苹果砧木叶绿素含量与光合速率研究[J].江苏农业科学, 2015, 43(3):149-150.
[23] 陈华蕊,陈业渊,高爱平,等.芒果叶绿素含量、比叶重与光合速率关系的研究[J].西南农业学报, 2010, 23(6):1848-1850.
[24] 李伶俐,房卫平,谢德意,等.不同品种杂交棉的光合特性及产量比较[J].中国农学通报,2006,22(9):189-192.
[25] 潘学标.不同叶色基因型棉花的一些光合特性比较[J].植物生理学通报,1989(5):20-23.
[26] Wang D, Sun S X, Gao F Y, et al. Mapping a rice glabrous gene using simple sequence repeat markers[J]. Rice Science, 2009, 16(2):93-98.
[27] Turley R B, Vaughn K C. Differential expression of trichomes on the leaves of upland cotton (Gossypium hirsutum L.)[J]. Molecular Biology and Physiology, 2012, 16:53-71.
[28] Ali I, Kausar A, Mehboob-ur-rehman M, et al. Development of genetic linkage map of leaf hairiness in Gossypium hirsutum (cotton) using molecular markers[J]. Pakistan Journal of Botany, 2009, 41(4):1627-1635.
[29] Desai A, Chee P W, May O L, et al. Correspondence of trichome mutations in diploid and tetraploid cottons[J]. The Journal of Heredity, 2008, 99(2):182-186.
[30] Lacape J M, Nguyen T B. Mapping quantitative trait loci associated with leaf and stem pubescence in cotton[J]. The Journal of Heredity, 2005, 96(4):441-444.
[31] 刘德新.陆地棉遗传图谱加密与T_1区域纤维品质QTL精细定位及候选基因鉴定[D].重庆:西南大学,2015.
[32] 赵亮.四倍体栽培棉种高密度遗传图谱的加密及棉花红株R1和茸毛T1基因的精细定位[D].南京:南京农业大学,2012.
[33] 武予清,郭予元.棉花单宁-黄酮类化合物对棉铃虫的抗性潜力[J].生态学报,2001,21(2):286-289.
[34] 刘国坤,谢联辉,林奇英,等.15种植物的单宁提取物对烟草花叶病毒(TMV)的抑制作用[J].植物病理学报,2003,33(3):279-283.
[35] 江汉民,王楠,赵换,等.花椰菜苯丙氨酸解氨酶基因的克隆及黑腐病菌胁迫下的表达分析[J].南开大学学报:自然科学版,2012,45(4):87-92.
[36] 董春娟,李亮,曹宁,等. 苯丙氨酸解氨酶在诱导黄瓜幼苗抗寒性中的作用[J]. 应用生态学报, 2015, 26(7):2041-2049.
[37] Macdonald M J, D'cunha G B. A modern view of phenylalanine ammonia lyase[J]. Biochemistry and Cell Biology, 2007, 85(3):273-282.
[38] 牛庆霖,冯殿齐,王玉山,等.盐碱胁迫对枣疯病枝过氧化物酶与苯丙氨酸解氨酶的研究[J].中国农学报,2010,26(2):100-104.

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