Overexpression of a WRKY Transcription Factor Gene from Juglans sigillata Dode Confers Resistance to Colletotrichum gloeosporioides in Transgenic Tobacco Plants

doi:10.7668/hbnxb.2016.03.023

Abstract

Abstract: To investigate the function of JsWRKY1, a constitutive expression vector of JsWRKY1 was constructed and transferred into Nicotiana tabacum L.cv Xanthi.There were no visible differences between the positive transgenic plants and WT.The expression levels of several defense-related genes MnSOD, Cu/ZnSOD, CN, NADPH oxidase, and PR1 resistance gene were up-regulated in the transgenic tobacco lines,and the SOD,APX and POD showed significantly higher activities in the transgenic lines than in wild type under normal conditions or after inoculation with C.gloeosporioides. The crude protein extract of transgenic tobacco lines inhibited the hyphal growth of the following four fungi, Botrosphaeria dothidea, Gibberella moniliformis, C.gloeosporioides and Fusarium oxysporum at different degrees.The antifungal activity in vitro plates demonstrated that the activities of SOD in the transgenic tobacco lines were significantly higher than those in WT.Moreover,the transgenic tobacco plants showed strong resistance after inoculation with C.gloeosporioides in the leaves.In conclusion,the JsWRKY1 is a positive transcription factor of J.sigiuata regulating the defense response to pathogens.

Key words: Juglans sigillata dode, WRKY transcription factor, Defense-related genes, Antioxidant enzyme, Colletotrichum gloeosporioides

CLC Number:

Q78
S436.62

WANG Guodong, CHEN Chaoyin, LI Jinjing, PU Limei, GUAN Ruipan, GE Feng, LIU Diqiu. Overexpression of a WRKY Transcription Factor Gene from Juglans sigillata Dode Confers Resistance to Colletotrichum gloeosporioides in Transgenic Tobacco Plants[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 155-162. doi: 10.7668/hbnxb.2016.03.023.

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