Cloning and Expression Analysis of a Potassium Transporter NtKT12 in Nicotiana tabacum

doi:10.7668/hbnxb.2016.02.012

Abstract

Abstract: Cloned the tobacco potassium transporter gene and predicted its structure, and analyzed their evolutionary relationships and functions, to lay the foundation for the study of tobacco potassium absorption mechanism.In this study, a homologous gene of a potassium transporter was cloned by RT-PCR from K326.Sequences analysis showed this gene contained 2 532 bp and coded 844 amino acid residues, and the calculated molecular mass was 93.1 kDa with the theoretical isoelectric point(pI) was 7.2.Homology analysis suggested that the gene had a high homology with the potassium transporter 12 of the Nicotiana tomentosiformis and Nicotiana sylvestris, so it was named as NtKT12.Protein structure and function analysis showed that NtKT12 has 12 transmembrane domains.Expression patterns showed that the expression level was the highest in the roots.Expression patterns under low potassium treatment suggested NtKT12 expression level was significantly higher after 6, 24 h than the control.These results provided some basis for potassium function analysis of NtKT12.

Key words: Tobacco, NtKT12, Cloning, Sequence analysis, Expression

CLC Number:

Q78
S572.03

LI Jiao, XU Li, LU Liming, LI Liqin. Cloning and Expression Analysis of a Potassium Transporter NtKT12 in Nicotiana tabacum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 65-70. doi: 10.7668/hbnxb.2016.02.012.

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http://www.hbnxb.net/EN/Y2016/V31/I2/65

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