Characteristics of Yak Tumor Protein p53 Gene(Tp53 )and Its Expression in Different Periods of Estrus Ovaries

doi:10.7668/hbnxb.2015.06.011

Abstract

Abstract: In order to research characteristics of yak Tp53 gene and its expression in different estrus period ovary.Gathered the ovarian during in different periods.The specific primers were on the basis of conservative Tp53 gene sequence of cattle,the gene sequence could be obtained by cloning,analyzed its gene structure and bioinformatics.Real-time PCR method for Tp53 gene expression in yak different estrus period ovary.The results showed Tp53 gene sequence coding region of 1 161 bp,and encoding 386 amino acids on yak.Homology and evolutionary analysis showed the highest homology with Tp53 gene,it could reach 98.4%,the lowest with domestic cats,80.7%,indicated that Tp53 gene was highly conserved in evolution;Real-time PCR detection results showed that the expression of Tp53 in each period was significant different( P<0.05).Tip:the reason why the expression of Tp53 was different might be due to the extent of DNA damage and endocrine factors in different estrus period ovaries.The successful cloning and bioinformatics analysis in yak Tp53 laid a foundation for the further study.

Key words: Bos grunniens, Tp53, Cloning, Bioinformatics, Ovary

CLC Number:

S823
Q78

ZHENG Hong-fei, PAN Yang-yang, LIU Peng-gang, PENG Xiu-mei, CUI Yan, YU Si-jiu. Characteristics of Yak Tumor Protein p53 Gene(Tp53 )and Its Expression in Different Periods of Estrus Ovaries[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(6): 70-76. doi: 10.7668/hbnxb.2015.06.011.

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References

[1] 陆仲磷,阎萍,王槐田.中国牦牛科学技术发展回顾与展望[J].中国草食动物,2003,23(3):33-36.
[2] 钟金城,陈智华.牦牛遗传育种学研究[J].云南畜牧兽医,2000(2):15-17.
[3] 刘忠贤,钱依宁.牦牛的遗传育种与改良[J].饲料与畜牧,1999(5):25-26.
[4] 周桔,罗荣保,汤长发,等.Bcl-2蛋白家族和p53 基因在细胞凋亡中的调控效应[J].中国组织工程研究与临床康复,2007,11(10):1950-1952.
[5] 舒明明.复发性流产绒毛组织中细胞凋亡信号通路的研究[D].石家庄:河北医科大学,2014.
[6] Levine A J,Oren M.The first 30 years of p53:growing ever more complex[J].Nature Reviews Cancer,2009,9(10):749-758.
[7] Lozano G,Zambetti G P.What have animal models taught us about the p53 pathway[J].The Journal of Pathology,2005,205(2):206-220.
[8] Yap D S,Hsieh J K,Zhong S,et al.Ser392 phosphorylation regulates the oncogenic function of mutant p53[J].Cancer Research,2004,64(14):4749-4754.
[9] Fridman J S,Lowe S W.Control of apoptosis by p53[J].Oncogene,2003,22(56):9030-9040.
[10] Hu W W.The role of p53 gene family in reproduction[J].Cold Spring Harbor Perspectives in Biology,2009,1(6):a001073.
[11] Yaron Y,Schwartz D,Evans M I,et al.p53 tumor suppressor gene expression in the mouse ovary during an artificially induced ovulatory cycle[J].The Journal of Reproductive Medicine,1999,44(2):107-114.
[12] 李瑞哲.p53干扰对牛体细胞核移植早期胚胎发育的影响[D].杨凌:西北农林科技大学,2013.
[13] Maruo T,Matsuo H,Samoto T,et al.Effects of progesterone on uterine leiomyoma growth and apoptosis[J].Steroids,2000,65(10/11):585-592.
[14] 卢翠玲,杨巍,胡召元,等.颗粒细胞的增殖分化及其在卵泡发育中的作用[J].科学通报,2005,50(21):2341-2347.
[15] Sirotkin A V,Benco A,Tandlmajerova A,et al.Transcription factor p53 can regulate proliferation,apoptosis and secretory activity of luteinizing porcine ovarian granulosa cell cultured with and without ghrelin and FSH[J].Reproduction,2008,136(5):611-618.
[16] 应诗家,彭中友,李燕,等.绵羊黄体期卵巢类固醇激素调节基因的表达研究[J].畜牧兽医学报,2013,44(11):1775-1780.
[17] Cherian-Shaw M,Das R,Vandevoort C A,et al.Regulation of steroidogenesis by p53 in macaque granulosa cells and H295R human adrenocortical cells[J].Endocrinology,2004,145(12):5734-5744.
[18] 李彩萍.生殖激素对体外培养的水牛卵泡颗粒细胞凋亡的影响研究[D].南宁:广西大学,2006.

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