New Method for Studying Plant Gene Function

doi:10.7668/hbnxb.2014.S1.023

Abstract

Abstract: TALEN(Transcription activator-like effector nuclease) CRISPR/Cas system(Clustered regularly interspaced short palindromic repeats,CRISPR.CRISPR-associated,Cas) have recently emerged as new technique for targeted genome modifications.They have been successfully applied in many fields such as animals,plants and microorganism.They can generate a double-strand break(DSB).Repair of these DSBs by intracellular DNA repair mechanisms enables to achieve the target gene modification.Compared with traditional method,TALEN and CRISPR/Cas system were stronger specificity,manipulated more easily and higher targeting efficiency.Here we report the construction,function and mechanism of the TALEN and CRISPR/Cas system.

Key words: Gene function, TALEN, CRISPR/Cas system

CLC Number:

PENG Chen, WANG Rui, LI Wan-chang, GUO Shi-wei. New Method for Studying Plant Gene Function[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 109-113. doi: 10.7668/hbnxb.2014.S1.023.

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http://www.hbnxb.net/EN/Y2014/V29/IS1/109

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