The Study on the Changes Cell Wall Components and Degrading Enzyme Activities in Flue-cured Tobacco during Bulk Curing Process

doi:10.7668/hbnxb.2010.04.035

Abstract

Abstract: The changes of main components of flue-cured tobacco cell walls and the activities of the cell wall degrading enzyme during flue-curing were researched by using the electric-heated flue curing barn designed and made by Henan Agricultural University. The contents of soluble pectin increased at first and then decreased while the contents of protopectin and cellulose decreased continuously during the bulk curing process. The activities of the cell wall degrading enzyme increased following with the process of flue-curing. The activities decreased after reaching to the peak value 48℃. The activities of pectinesterase(PE) was closely related with polygalacturonase(PG) . The interaction of PE and PG promoted the degradation of pectin. The action of Cellulase was mainly promoted the cellulose’s degradation. The degradation of cell wall components resulted from the synthetic action of the cell wall degrading enzyme.

Key words: Bulk curing, Flue-cured tobacco, Pectin, Cellulose, Cell wall-degrading enzyme

CLC Number:

S572

HUO Kai-ling, SONG Zhao-peng, ZHANG Wei-jian, WU Sheng-jiang, YU Jin-heng, GONG Chang-rong. The Study on the Changes Cell Wall Components and Degrading Enzyme Activities in Flue-cured Tobacco during Bulk Curing Process[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 166-169. doi: 10.7668/hbnxb.2010.04.035.

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http://www.hbnxb.net/EN/Y2010/V25/I4/166

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